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Thermo Scientific™ T4 DNA ligasi
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Marca: Thermo Scientific™ EL0012
284.78 EUR valido fino al 2024-12-31
Usa il codice promo "21884" per avere il tuo prezzo promozionale.
Dettagli aggiuntivi : Peso : 0.04990kg
Descrizione
Catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
The T4 DNA Ligase requires ATP as a cofactor.
Highlights
- Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
- Fast – sticky-end ligation is completed in 10 minutes at room temperature
- Supplied with PEG solution for efficient blunt-end ligation
Applications
- Cloning of restriction enzyme generated DNA fragments
- Cloning of PCR products
- Joining of double-stranded oligonucleotide linkers or adaptors to DNA
- Site-directed mutagenesis
- Amplified fragment length polymorphism (AFLP)
- Ligase-mediated RNA detection (see Reference 3)
- Nick repair in duplex DNA, RNA or DNA/RNA hybrids
- Self-circularization of linear DNA.
Notes
- Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis.
- The volume of the ligation reaction mixture should not exceed 10% of the competent cell volume in the transformation process.
- Prior to electro-transformation, remove T4 DNA Ligase from the ligation mixture by spin column or chloroform extraction. The extracted DNA can be further precipitated with ethanol.
Specifica
| 5 U/μL | |
| 10X T4 DNA Ligase Buffer | |
| T4 DNA Ligase |
| DNA Ligase | |
| 5 x 1000U |
Sicurezza e movimentazione
Identificativo prodotto
Avvertenza
Categoria di pericolo
Indicazioni di pericolo
Consigli di prudenza
Informazioni supplementari
- T4 DNA Ligase (5 U/µL)
Avvertenza
- Attenzione
Categoria di pericolo
- Lesione oculare grave/irritazione oculare Categoria 2
Indicazioni di pericolo
- H319-Provoca grave irritazione oculare.
Consigli di prudenza
- P264-Lavare accuratamente dopo l'uso.
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
- P337+P313-Se l'irritazione degli occhi persiste, consultare un medico.
Informazioni supplementari
- MIXTURE LIST-Contiene: (R*,R*)-1,4-dimercapto-2,3-butanediol
For Research Use Only. Not for use in diagnostic procedures.