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Thermo Scientific™ 1-Step CHO High-Yield IVT Kit

Express of functional proteins with much greater yield per mL of reaction than rabbit reticulocyte lysate-based mammalian IVT systems using the Thermo Scientific™ 1-Step CHO High-Yield In Vitro Translation (IVT) Kit.

Marca:  Thermo Scientific™ 88893

Dettagli aggiuntivi : Peso : 0.10000kg

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Codice prodotto. 15392607

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Descrizione

Descrizione

The Thermo Scientific 1-Step CHO High-Yield In Vitro Translation (IVT) Kit enables the expression of functional proteins with up to 750 times greater yield per mL of reaction than rabbit reticulocyte lysate-based mammalian IVT systems that only yield up to 10μg protein/mL reaction.

  • High expression - up to 750μg/mL of expressed protein
  • Reproducible results - low variability between experiments
  • Fast - express high levels of protein with 6 hours to overnight incubation
  • Easier storage - allows you to store lyophilized CHO cell lysate at -20°C
  • Functional - obtain functionally active proteins, including those containing disulfide bonds
  • Easy-to-use - three reaction components together carry out transcription and translation in one step
  • Great for labeling - amenable to incorporation of labeled and unnatural amino acids
  • Vector compatibility - uses existing pT7CFE1 vectors for protein expression
The 1-Step CHO High-Yield IVT System uses extracts containing the robust translation machinery of the pharmaceutical industry standard Chinese Hamster Ovary (CHO) cell line to generate functional full-length proteins. In this system, protein expression is performed in a proprietary dialysis device that allows a continuous supply of nucleotides, amino acids, and energy-generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression in an overnight incubation of up to 750μg/mL. The CHO IVT kit includes all of the components required for transcription and translation of a recombinant gene, including an optimized expression vector.
Includes:
Lyophilized cell lysate, reaction mix, accessory proteins, 4X dialysis buffer, control DNA, cloning vector, dialysis devices, and centrifuge tubes.
Requires:
30°C incubator or water bath (shaker incubator increases expression by 30 to 50%)
Applications:
  • Production of large amounts of recombinant protein for structural analysis
  • Generation of active proteins for downstream applications such as protein interaction studies
  • Rapid large-scale expression and characterization of mutant proteins
  • Production of viral particles
  • Incorporation of unnatural amino acids
  • Production of cytotoxic proteins

Specifica

Specifica

Protein biology, protein expression, cell-free (In-vitro) expression, In-vitro translation, production of large amounts of recombinant protein for structural analysis, generation of active proteins for downstream applications such as protein interaction s
Multi-component kit
1-Step™ CHO high-yield IVT Kit
2 x 100μL
2 reactions of 100 μL each, 2 Reactions of 100μL each
1-Step™ CHO High-Yield IVT Kit, 2 x 100μL, Cell-Free Expression, Sufficient for 2 Reactions Of 100μL Each, –68 to –85°C Store In Ultra-Cold Freezer, Production of Large Amounts of Recombinant Protein for Structural Analysis
Lyophilized CHO lysate 1 vial, accessory proteins 25μL, dialysis buffer (4X) 900μL, reaction mix 40μL, positive control DNA (pCFE-GFP) 10μg, pT7CFE1-NHis-GST-CHA 10μg, microdialysis device 2 each, nuclease-free water 50mL
Lyophilized CHO lysate 1 vial, accessory proteins 25μL, dialysis buffer (4X) 900μL, reaction mix 40μL, positive control DNA (pCFE-GFP) 10μg, pT7CFE1-NHis-GST-CHA 10μg, microdialysis device 2 each, nuclease-free water 50mL
Store in ultra-cold freezer (-68 to -85°C)., –68 to –85°C
750 Times greater yield per mL
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