Thermo Scientific™ Lysozyme

Lysozyme is an enzyme used to break down bacterial cell walls to improve protein or nucleic acid extraction efficiency. Lysozymes (muramidases) are a family of enzymes with antimicrobial activity characterized by the ability to damage the cell wall of bacteria. The enzyme acts by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycans and between the N-acetyl-D-glucosamine residues in chitodextrins. Although hen egg white lysozyme is most effective for the lysis of gram-positive bacteria, it also facilitates the lysis of gram-negative bacteria such as Salmonella and Shigella. The lysis of E. coli is especially improved by the addition of both lysozyme and a nucleases such as DNase I.

Highlights:

• Bacterial cell wall lytic enzyme that improves inclusion body protein purification
• Compatible with Thermo Scientific Pierce Cell Lysis Reagents
• Available in two convenient formats: lyophilized powder for large volume applications (Part No. 89833) and convenient glycerol freezer stock (Part No. 90082)

Properties of lysozyme:

• Enzymatic function: Catalyzes the hydrolysis of 1,4-β-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycans and between N-acetyl-D-glucosamine residues in chitodextrins
• Molecular weight: 14.388 kDa
• Extinction coefficient: ∽26.4 at 280nm
• Isoelectric point: pH 11.0
• Unit definition: 1 unit is that amount of enzyme needed to catalyze a decrease in absorbance at 450nm of 0.001/min at 25 degrees at pH 6.24 in a 1cm cuvette due to lysis using a ∼0.25mg/mL suspension of Micrococcus luteus.