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Thermo Scientific™ pEF1α Tluc16-DD Vector for Luciferase Assays

Codice prodotto. 15362637 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
10 μg
Dimensione della confezione:
10µg
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15362637 10 μg 10µg
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Codice prodotto. 15362637 Fornitore Thermo Scientific™ N. del fornitore 88241

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Descrizione

Insert promoter sequences for genes of interest into Thermo Scientific™ Tluc16 Vectors to measure gene regulation using the intracellular TurboLuc™ 16 (Tluc16) luciferase reporter in luciferase assays.

The Thermo Scientific pMCS Tluc16 Vectors are multiple cloning site (MCS) vectors designed to accept a promoter sequence for study of gene regulation using the intracellular TurboLuc™16 (Tluc16) luciferase reporter.

  • Intracellular Tluc16 luciferase gene, optimized for high expression in mammalian systems
  • Multiple cloning sites provide versatility for transfer of regulatory elements from one vector to another
  • Synthetic polyA terminator and Transcriptional Pause Site (TPS) included upstream of MCS to minimize non-specific transcriptional read-through
  • Dual-destabilization (DD) technology reduces accumulation of Tluc16 luciferase mRNA and protein in cells, enhancing the responsiveness of the assay
  • Optimized minimal core promoter (minP) and 5' UTR region for efficient expression of Tluc16 luciferase
  • Hygromycin resistance gene for use as selection marker to generate mammalian stable cell lines
  • Prepared pNF-κB, and pCRE Tluc16-DD Vectors are designed to monitor the activation of NFκB protein and cAMP-binding protein (CREB) in mammalian cells
  • Prepared pEF1α variety includes a modified version of the Elongated Factor 1α (EF1α) promoter for constitutive Tluc16 luciferase expression in mammalian cells
Tluc16 luciferase is a 16 kDa, novel, intracellular luciferase derived from the marine copedod Metridia luciferase family. The wild-type luciferase has been modified to reduce its size, increase its brightness, and enable its intracellular expression. A synthetic polyA terminator and a Transcriptional Pause Site (TPS) are included upstream of the MCS to minimize non-specific transcriptional read-through. The Tluc16 luciferase activity can be measured using the Thermo Scientific TurboLuc™ One-Step Glow Assay Kit.

TRUSTED_SUSTAINABILITY

Specifica

Tipo Transcriptional Reporter Vector
Cloning Method Restriction Enzyme ⁄ MCS
Tipo di erogazione Transfection
Quantità 10 μg
Content And Storage Store in freezer (-5 to -30°C).
Gene reporter TurboLuc™16 Luciferase
Da utilizzare con (applicazione) Reporter Assays
Promotore EF-1α

For Research Use Only. Not for use in diagnostic procedures.

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