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Thermo Scientific™ Kit di trasformazione batterica TransformAid

Codice prodotto. 10344730 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
40 Transformations
Dimensione della confezione:
40 reazioni
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10344730 40 Transformations 40 reazioni
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Codice prodotto. 10344730 Fornitore Thermo Scientific™ N. del fornitore K2711

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Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price

Kit for quick and convenient preparation of competent E.coli cells for guaranted transformation efficiencies of more than 107 transformants per μg of plasmid DNA.

Employ a novel method for rapid preparation of chemically-competent E. coli cells from overnight bacterial cultures or bacterial colonies with the Thermo Scientific TransformAid™ Bacterial Transformation Kit. The key component of this system is the unique T-solution, which produces competent cells in a few easy steps. The quick and convenient procedure guarantees transformation efficiencies of more than 107 transformants per μg of plasmid DNA, ideal for routine cloning experiments. The option to use bacterial colonies for preparation of competent cells adds flexibility to any cloning experiment.

The TransformAid Bacterial Transformation Kit can be used with most E. coli strains commonly used for cloning: JM107, JM109, XL1-Blue, SURE, TOPP2, W3110, NM527, AD494, CJ236, GM2163, C600, BL21, M15, NM522, HB101, ER2267.

Highlights

  • Efficient – more than 107 transformants per μg of plasmid DNA
  • Practical – E. coli competent cells can be prepared from an overnight culture or from bacterial colonies
  • Fast – less than 1 hour from the overnight bacterial culture to cell plating. Starting from bacterial colonies, the procedure requires only 2.5 hours
  • Easy – all procedures are performed on ice on your bench. Brief centrifugations are carried out in regular microcentrifuges

Applications

  • Routine cloning experiments
  • Blunt-end cloning
  • TA cloning

Includes

  • C-Medium
  • T-Solution (A)
  • T-Solution (B)
  • Detailed Protocol

Note

Competent cells prepared with TransformAid Bacterial Transformation Kit are suitable for direct-use only. Freezing down and storage at -70°C is not recommended.

TRUSTED_SUSTAINABILITY

Specifica

Tipo di cellula E. coli
Formato Kit
Specie E. coli
Efficienza ≥1 x 107;
Da utilizzare con (applicazione) Cloning
Linea di prodotti TransformAid
Product Type TransformAid Bacterial Transformation Kit
Quantità 40 Transformations

For Research Use Only. Not for use in diagnostic procedures.

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