Thermo Scientific™ Treponema pallidum Haemagglutination Test Kit (TPHA)

Syphilis is a sexually transmitted disease. The causative organism is Treponema pallidum, a spirochete which cannot be grown on culture media or in tissue culture. Diagnosis of infection is normally by the detection of antibody specific for Treponema pallidum in the patient’s blood or CSF.

The TPHA test kit is a sensitive passive haemagglutination test specifically designed for the detection of antibodies to Treponema pallidum.

• Rapid: within little over 30 minutes of receiving the test sample or culture
• Simple: ready-to-use reagents
• Easy-to-read results
• Excellent sensitivity: high quality specific antigens used in the test provide excellent sensitivity.

Tanned fowl erythrocytes are coated with specific antigen and suspended in a diluent ^1,2,3. When diluted positive samples are mixed with the test suspension, antibody to the sensitising antigen causes agglutination of the cells. The cells form a characteristic pattern in the bottom of a microtitration plate well. In the absence of reacting antibody, the cells form a compact button in the well. Uncoated tanned fowl erythrocytes are used as the control cells.

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