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Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Reagents and Kits (128)
Real Time PCR Master Mixes (122)
Real Time PCR Assays and Arrays (11)

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1 – 15 di 152 risultati
1

Applied Biosystems™ MycoSEQ™ Mycoplasma Detection Kits

The MycoSEQ Mycoplasma Detection Kit is a real-time mycoplasma PCR assay designed and validated to meet the requirements of European Pharmacopeia Chapter 2.6.7 for use as a nucleic acid amplification technique (NAT).

EDGE
Quantità 100 Reactions
Label or Dye SYBR Green
Da utilizzare con (applicazione) Mycoplasma Detection
Descrizione Detection for BioProduction process media, cells, raw materials
Linea di prodotti MycoSEQ
2

Applied Biosystems™ ViralSEQ™ Mouse Minute Virus (MMV) Detection System

The Applied Biosystems ViralSEQ Mouse Minute Virus (MMV) Detection Assay is part of an integrated DNA purification and real-time PCR-based system for rapid, sensitive detection of MMV from a wide range of sample types.

EDGE
3

Applied Biosystems™ resDNASEQ™ Quantitative Sf9 and Baculovirus DNA Kits

The resDNASEQ Quantitative Sf9 and Baculovirus DNA Kit is a duplex quantitative PCR (qPCR)-based system for the detection of residual DNA from the Sf9 baculovirus platform.

EDGE
Quantità 100 reactions
Metodo di rilevamento Primer-probe
Label or Dye FAM + VIC
N. di reazioni 100 Reactions
Linea di prodotti resDNASEQ
Host Cell SF9 + Bacoluvirus
PCR Method qPCR
Formato Kit
4

Applied Biosystems™ resDNASEQ™ Quantitative HEK293 DNA Kits

The resDNASEQ Quantitative HEK293 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host-cell DNA from HEK293 cell lines.

EDGE
Quantità 100 reactions
Metodo di rilevamento Primer-probe
Label or Dye FAM
N. di reazioni 100 Reactions
Linea di prodotti resDNASEQ
Host Cell HEK
PCR Method qPCR
Formato Kit
5

Applied Biosystems™ MycoSEQ™ Plus Mycoplasma Detection Kits

The MycoSEQ™ Plus Mycoplasma Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for the detection of mycoplasma in complex bioproduction samples.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance Medium
Forma Liquid
Metodo di rilevamento Primer-probe
Product Type Mycoplasma Detection
Reaction Speed Standard
Label or Dye FAM
N. di reazioni 100 Reactions
Da utilizzare con (applicazione) Microbial Detection
Linea di prodotti MycoSEQ
Tipo di campione Biopharmaceutical Purification Sample, Bulk Drug Substance Sample, Cell Cultures
PCR Method Multiplex qPCR
Target Organism Class Mollicutes, mycoplasma
6

Applied Biosystems™ resDNASEQ™ Quantitative E. coli DNA Kits

This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E. coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than 4 hours.

Quantità 100 reactions
Metodo di rilevamento Primer-probe
Label or Dye FAM
N. di reazioni 100 Reactions
Linea di prodotti resDNASEQ
Host Cell E.coli
PCR Method qPCR
Formato Kit
7
Promozioni disponibili

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

Purity or Quality Grade UP (Ultra Pure)
Polimerasi AmpliTaq Gold DNA Polymerase
Linea di prodotti TaqMan
PCR Method qPCR
Formato Bottle
Da utilizzare con (apparecchiatura) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Metodo di rilevamento Primer-probe
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Reaction Speed Standard
Hot Start Built-In Hot Start
Da utilizzare con (applicazione) Gene Expression,Genotyping,Pathogen Detection,Virus Detection
Tipo di campione Environmental Sample, Food
Concentrazione 2X
9

Fisherbrand™ SYBR™ Green qPCR Master Mix

Fisherbrand™ SYBR™ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Content And Storage SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Forma Frozen
Metodo di rilevamento SYBR
Product Type Master Mix
Reaction Speed Fast or Standard
Label or Dye SYBR Green,FAM
Da utilizzare con (applicazione) Gene Expression,DNA Quantitation,Microbial Detection,Real Time PCR (qPCR)
PCR Method qPCR,2-step RT-qPCR,RT-qPCR
Concentrazione 2X
10

Applied Biosystems™ resDNASEQ™ Quantitative CHO DNA Kits

This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ Quantitative CHO DNA System, allows users to detect sub-picogram amounts of residual DNA from CHO cell line in under 5 hours.

Quantità 100 reactions
Metodo di rilevamento Primer-probe
Label or Dye FAM
N. di reazioni 100 Reactions
Linea di prodotti resDNASEQ
Host Cell CHO
PCR Method qPCR
Formato Kit
11

Applied Biosystems™ resDNASEQ™ Quantitative Vero DNA Kits

The resDNASEQ Quantitative Vero DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Vero (African Green Monkey) cells, an expression system commonly used for the production of vaccines.

Quantità 100 reactions
Metodo di rilevamento Primer-probe
Label or Dye FAM
N. di reazioni 100 Reactions
Linea di prodotti resDNASEQ
Host Cell Vero
PCR Method qPCR
Formato Kit
12

Applied Biosystems™ resDNASEQ™ Human Residual DNA Quantitation Kits

The resDNASEQ Human Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from human cell lines used as expression systems for recombinant proteins.

Quantità 100 reactions
Metodo di rilevamento Primer-probe
Label or Dye FAM
N. di reazioni 100 Reactions
Linea di prodotti resDNASEQ
Host Cell Human DNA
PCR Method qPCR
Formato Kit
13
Promozioni disponibili

Applied Biosystems™ TaqMan™ TAMRA Probe
GREENER_CHOICE

Custom TaqMan™ Probe

Shipping Condition Room Temperature
Da utilizzare con (apparecchiatura) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Content And Storage 1 tube containing a dual-labeled probe in solution or dried down.

Store at -15°C to -25°C
Purity or Quality Grade HPLC
Forma Liquid or Dried Down
Internal Probe Modification None
Product Type TAMRA Probe
5'Primer Modification FAM, TET, VIC
Da utilizzare con (applicazione) Real Time PCR (qPCR)
Metodo di purificazione HPLC
Linea di prodotti TaqMan
3'Primer Modification TAMRA
Formato Tube
14

Thermo Scientific™ SureTect ™ Vibrio cholerae, V parahaemolyticus and V. vulnificus PCR Assay

Rapidly detect Vibrio cholerae, V. parahaemolyticus and V. vulnificus in food samples in as little as 10 hours with this highly-specific, simple-to-use real-time PCR Assay.

15

Thermo Scientific™ ROX Solution, 50 μM

ROX dye is added to the qPCR reactions to serve as an internal reference for normalization of the fluorescent signal when using real-time instruments that can detect ROX.