PCR Supplies

PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.
Standard PCR Products (263)
Real Time PCR Products (185)
Reverse Transcription Products (38)
dNTPs (37)

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1 – 30 di 276 risultati
1

Cytiva Sfere illustra™ PuReTaq Ready-To-Go™ PCR

Biglie di reazione pre-miscelate e pre-dosate per eseguire amplificazioni PCR standard.  Le biglie Cytiva Lifescience™ illustra™ PuReTaq Ready-To-Go™ PCR sono stabili a temperatura ambiente.

Promozioni disponibili
EDGE
Quantità 1 Kit
Content And Storage Perle stabili a temperatura ambiente contenenti ∼ 2.5 unità di polimerasi del DNA PuReTaq ricombinante, dATP, dCTP, dGTP, dTTP, stabilizzatori, BSA e tampone di reazione e, quando una perla viene ricostituita al volume finale di 25 μl, la concentrazione di ciascun dNTP
Da utilizzare con (applicazione) Standard PCR
2

Thermo Scientific™ Kit di sintesi di cDNA a singolo filamento RevertAid H Minus

Perform efficient synthesis of first strand cDNA from RNA templates with this complete synthesis system.

Promozioni disponibili
Shipping Condition Dry Ice
Tecnica Reverse Transcription
Reverse Transcriptase RevertAid H Minus
Tipo di reagente Reverse Transcription
Reaction Format Separate components
Reaction Speed 60 min.
Ribonuclease H Activity Reduced
Da utilizzare con (applicazione) Real Time PCR (qPCR),RT-PCR
Optimal Reaction Temperature 42°C to 45°C
Starting Material RNA
Tipo di prodotto finale First-Strand cDNA
Dimensioni (prodotto finale) Up to 13 kb
Formato Kit
3

Thermo Scientific™ Kit qPCR verde DyNAmo™ HS SYBR™

Contains a modified Thermus brockianus DNA polymerase and all the other reagents needed for qPCR.

Promozioni disponibili
Da utilizzare con (apparecchiatura) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler
GC-Rich PCR Performance High
Metodo di rilevamento SYBR
Product Type Real Time PCR SYBR Master Mix
Reaction Speed Standard
Tipo di kit DyNAmo HS SYBR Green qPCR Kit
Da utilizzare con (applicazione) Gene Expression
Polimerasi Tbr DNA polymerase
Linea di prodotti DyNAmo
Tipo di campione DNA, dsDNA
PCR Method qPCR
Concentrazione 2X (DyNAmo HS SYBR Green qPCR Mix), 50X (ROX Dye)
Formato Tube
4

Thermo Scientific™ Taq DNA Polymerase, native, with BSA (5 U/µL)

Optimize routine PCR with this highly thermostable, native DNA polymerase.

5

Thermo Scientific™ dTTP

Optimize molecular biology applications with this extremely pure dTTP.

6

Thermo Scientific™ DNA polimerasi Taq, nativo

Optimize amplification of DNA samples of low purity with this highly thermostable, native DNA polymerase for routine PCR.

7

Thermo Scientific™ DreamTaq™ Green Buffer (10X)

A proprietary PCR buffer which contains KCl and (NH&subscript_4;)&subscript_2;SO&subscript_4; at a ratio optimized for robust performance of DreamTaq DNA Polymerase in PCR and includes MgCl&subscript_2;.

8

Thermo Scientific™ dCTP

Optimize molecular biology applications with this extremely pure dCTP.

9

Thermo Scientific™ Kit di sintesi di cDNA a singolo filamento RevertAid™ Premium

Perform efficient synthesis of first strand cDNA from RNA templates with this complete synthesis system.

Promozioni disponibili
Shipping Condition Dry Ice
Tecnica Reverse Transcription
Content And Storage

• RevertAid Reverse Transcriptase
• RiboLock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Oligo(dT)18 Primer
• Random Hexamer Primer
• Control GAPDH RNA
• Forward GAPDH Primer (10 μM)
• Reverse GAPDH Primer (10 μM)
• Nuclease-free water

Store at –20°C.
Reverse Transcriptase RevertAid
Tipo di reagente Reverse Transcription
Tipo di prodotto finale First-Strand cDNA
Dimensioni (prodotto finale) Up to 13 kb
Formato Kit
Reaction Format Separate components
Reaction Speed 60 min.
Ribonuclease H Activity Yes
Da utilizzare con (applicazione) RT-PCR
Optimal Reaction Temperature 42°C
Starting Material RNA
10

Thermo Scientific™ dITP Solution (100 mM)

Optimize molecular biology applications with this extremely pure dITP.

11

Thermo Scientific™ Kit per purificazione PCR GeneJET™

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Promozioni disponibili
Compatibilità ad alta produttività Not High-throughput Compatible (Manual)
Run Time 5 min.
Formato Spin Column
12

Thermo Scientific™ MuA Transposase (concentrated)

Perform functional analysis of proteins with these transposon-based tools.

13

Thermo Scientific™ Aminoallyl-dUTP Solution (10 mM)

Perform enzymatic indirect non-radioactive labeling of DNA with the modified nucleotide Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate).

14

Thermo Scientific™ dGTP

Optimize molecular biology applications with this extremely pure dGTP.

15

Thermo Scientific™ Luminaris Color Probe qPCR Master Mix, high ROX

Ready-to-use qPCR master mixes with color and premixed UDG optimized for probe detection using standard cycling protocols.

Promozioni disponibili
Da utilizzare con (apparecchiatura) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
GC-Rich PCR Performance High
Metodo di rilevamento Primer-Probe
Product Type qPCR Master Mix
Reaction Speed Standard
Da utilizzare con (applicazione) Gene Expression,Genotyping,siRNA Validation
Polimerasi Taq DNA Polymerase
Descrizione Color Probe High ROX
Linea di prodotti Luminaris
Tipo di campione DNA, dsDNA
PCR Method qPCR
Concentrazione 2X (Luminaris Color Probe High ROX qPCR Master Mix), 40X (Yellow Sample Buffer)
Formato Tube
16

Thermo Scientific™ ROX Solution, 50 µM

ROX dye is added to the qPCR reactions to serve as an internal reference for normalization of the fluorescent signal when using real-time instruments that can detect ROX.

17

Thermo Scientific™ Luminaris Color Probe qPCR Master Mix

Optimize qPCR with probe detection using standard cycling protocols with these ready-to-use master mixes with premixed UDG, available with or without blue color.

Promozioni disponibili
Da utilizzare con (apparecchiatura) BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
GC-Rich PCR Performance High
Metodo di rilevamento Primer-Probe
Product Type qPCR Master Mix
Reaction Speed Standard
Da utilizzare con (applicazione) Gene Expression
Polimerasi Taq DNA Polymerase
Descrizione Color Probe
Linea di prodotti Luminaris
Tipo di campione DNA, dsDNA
PCR Method qPCR
Concentrazione 2X (Luminaris Color Probe qPCR Master Mix), 40X (Yellow Sample Buffer)
Formato Tube
18

Thermo Scientific™ Miscela master per PCR Phusion Blood Direct

Amplify DNA directly from whole blood without prior purification

GC-Rich PCR Performance High
Includes Label or Dye Yes
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Polimerasi Phusion Blood II DNA Polymerase
Linea di prodotti Phusion
Tipo di campione Blood
Starting Material DNA
PCR Method Direct PCR
Sporgenza Blunt
19

Thermo Scientific™ Kit per PCR diretta per tessuti animali Phire™

PCR directly from various animal tissues including mice, fish, birds and insects without DNA purification.

20

Thermo Scientific™ Phusion U Green Hot Start DNA Polymerase

Thermo Scientific Phusion U DNA Polymerase is a proofreading enzyme (25x Taq) with a proprietary mutation to read through uracils and incorporate dUTP

GC-Rich PCR Performance High
Content And Storage Includes:
• Phusion U Hot Start DNA Polymerase, 2 U/µL
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO
Both Phusion Green HF Buffer and Phusion Green GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.

Store at -20°C.
Polimerasi Phusion U Hot Start DNA Polymerase
Linea di prodotti Phusion
Dimensioni (prodotto finale) 20 kb
Fedeltà (rispetto a taq) 25 X
Formato Tube
Reaction Format Standalone
Product Type Hot Start DNA Polymerase
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
Sporgenza Blunt
Concentrazione 2 U/µL
21

Thermo Scientific™ Phusion Green Hot Start II High-Fidelity DNA Polymerase (2 U/µL)

The most accurate hot start DNA polymerase on the market featuring extreme specificity and improved performance.

GC-Rich PCR Performance High
Content And Storage Includes:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO
• 50 mM MgCl2 solution

Both Phusion Green HF and GC Buffers provide 1.5 mM MgCl2 in the final 1X concentration.Store at -20°C.
Reaction Format Standalone
Reaction Speed Fast
Hot Start Built-In Hot Start
Polimerasi Phusion Hot Start II High-Fidelity DNA Polymerase
Linea di prodotti Phusion
Starting Material DNA
Sporgenza Blunt
Concentrazione 2 U/µL
Dimensioni (prodotto finale) 20 kb or Less
Fedeltà (rispetto a taq) 52 X
Formato Tube
22

Thermo Scientific™ Phusion Green GC Buffer Pack

Buffer packs for use in PCR with Phusion High-Fidelity DNA Polymerases

23

Thermo Scientific™ Luminaris HiGreen qPCR Master Mix

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Promozioni disponibili
Da utilizzare con (apparecchiatura) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
GC-Rich PCR Performance High
Metodo di rilevamento SYBR
Product Type qPCR Master Mix
Reaction Speed Standard
Da utilizzare con (applicazione) Gene Expression
Polimerasi Taq DNA Polymerase
Linea di prodotti Luminaris
Tipo di campione DNA, dsDNA
PCR Method qPCR
Concentrazione 2X
Fedeltà (rispetto a taq) 2 X
24

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, ROX

Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Promozioni disponibili
Da utilizzare con (apparecchiatura) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
GC-Rich PCR Performance High
Metodo di rilevamento SYBR
Product Type Real Time PCR SYBR Master Mix
Reaction Speed Standard
Da utilizzare con (applicazione) Gene Expression,Genotyping,siRNA Validation
Polimerasi Taq DNA Polymerase
Linea di prodotti ABsolute Blue
Tipo di campione DNA, dsDNA
Compatibilità qPCR Cyclers
PCR Method qPCR
Concentrazione 2X
Formato Tube
25

Thermo Scientific™ ABsolute Blue QPCR Mix, low ROX

ABsolute Blue qPCR Mixes are 2X master mixes for probe chemistry. There are suitable alternatives available for all common real-time instruments.

Promozioni disponibili
Da utilizzare con (apparecchiatura) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
GC-Rich PCR Performance High
Metodo di rilevamento Primer-Probe
Product Type qPCR Mix
Reaction Speed Standard
Da utilizzare con (applicazione) Gene Expression
Polimerasi Taq DNA Polymerase
Linea di prodotti ABsolute Blue
Tipo di campione DNA, dsDNA
PCR Method qPCR
Concentrazione 2X
Fedeltà (rispetto a taq) 2 X
Formato Tube
26

Thermo Scientific™ DreamTaq Green PCR Master Mix (2X)

Save time in PCR applications with Master Mix and get higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase.

GC-Rich PCR Performance Low
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Hot Start No
Polimerasi DreamTaq DNA Polymerase
Linea di prodotti DreamTaq™
Sporgenza 3'-A
Fedeltà (rispetto a taq) 1 X
27

Thermo Scientific™ ABsolute™ qPCR Mixes

ABsolute qPCR Mixes are optimized for use with hybridization probes.

Promozioni disponibili
28

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, low ROX

Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Promozioni disponibili
Da utilizzare con (apparecchiatura) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
GC-Rich PCR Performance High
Metodo di rilevamento SYBR
Product Type Real Time PCR SYBR Master Mix
Reaction Speed Standard
Da utilizzare con (applicazione) Gene Expression,Genotyping,siRNA Validation
Polimerasi Taq DNA Polymerase
Linea di prodotti ABsolute Blue
Tipo di campione DNA, dsDNA
Compatibilità qPCR Cyclers
PCR Method qPCR
Concentrazione 2X
Formato Tube
29

Thermo Scientific™ DNA polimerasi DreamTaq Green

Save time in standard PCR applications with this enhanced Taq DNA polymerase, supplied with colored buffer enabling direct gel loading.

30

Thermo Scientific™ ABsolute QPCR Mix, no ROX

Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

Promozioni disponibili
Da utilizzare con (apparecchiatura) BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Metodo di rilevamento Primer-Probe
Reaction Speed Standard
Polimerasi Taq DNA Polymerase
Linea di prodotti ABsolute
Tipo di campione DNA, dsDNA
PCR Method qPCR