Protein Purification
Cytiva Colonne preimpaccate HisTrap™ HP
Per la purificazione diretta di proteine marcate con istidina da cellule non chiarificate omogeneizzate. Le colonne Cytiva Life Sciences™ HisTrap™ HP sono preimpaccate con Ni Sepharose™ High Performance e consentono la facile purificazione in un solo passaggio di proteine marcate con istidina tramite cromatografia di affinità con ioni metallici immobilizzati (IMAC).
Cytiva Colonne per la preparazione dei campioni PD MiniTrap™ G-25
Per pulire biomolecole con peso molecolare di >5000 MR in volumi di campioni fino a 0.5 ml. Le colonne per preparazione di campioni Cytiva Life SciencesTM D MiniTrapTM G-25 sono pratiche e preimpaccate.
Thermo Scientific™ Cocktail inibitori della proteasi Halt™
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.
Thermo Scientific™ Lisozima Pierce™
Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.
Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit
Chromogenic Endotoxin Quant Kits are highly sensitive endpoint assays that measure and detect endotoxin (LPS) in protein, peptide, nucleic acid, or antibody samples using the Amebocyte Lysate Assay from 0.01–1.0 EU/mL. Kits are available in reaction sizes ranging from 30 to 240.
Thermo Scientific™ HisPur™ Ni-NTA Spin Plates
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ Cocktail inibitore della fosfatasi Halt™
Stabilize serine, threonine and tyrosine phosphorylation of proteins in cells and tissues with this cocktail of phosphatase inhibitors.
Thermo Scientific™ Bigliemagnetiche alla proteina AG Pierce™
Immunoprecipitate or affinity purify antibodies (IgG) using Protein A/G coated magnetic particles for manual or robotic magnetic separation.
Thermo Scientific™ Colonne di centrifugazione Pierce™
Pack these empty 0.5 to 10 mL spin columns with agarose beads to perform small-scale affinity purification methods in 15- or 50-mL conical microcentrifuge tubes.
Thermo Scientific™ CHAPS Detergent (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate)
Non-denaturing Zwitterionic detergents that disrupt nonspecific protein interactions with less protein aggregation than nonionic detergents.
Thermo Scientific™ Brij™-35, 30% Solution
Stabilized Brij-35 detergent provided as 10% and 30% solutions for use in various protein methods including cell lysis and HPLC.
Thermo Scientific™ Kit di frazionamento subcellulare di proteine per cellule in coltura
Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.
Thermo Scientific™ Pierce™ D-Galactose Agarose
Bind and affinity-purify lectins, galactosidases and other galactose-binding molecules using this beaded agarose resin with immobilized thio-alpha-D-galactose.
Thermo Scientific™ Colonne in plastica monouso Pierce™
Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.
Thermo Scientific™ Resina HisPur™ Ni-NTA
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.