PCR and qPCR

Polymerase chain reaction (PCR) and quantitative PCR (qPCR) are methods used to make copies of genetic material so that small amounts can be tested. Whether you are cloning DNA, conducting mutagenesis experiments, performing forensic or parental testing, or detecting pathogens, you can find the primers, polymerases, master mixes, dyes, and thermal cyclers you need to complete your workflow.

Overview

1. Sample Preparation

2. PCR Amplification

3. qPCR Reverse Transcription

4. qPCR Quantification

5. Essential Supplies

Related Resources

Other Applications

PCR and qPCR

1. Sample Preparation

Preparing samples for PCR or qPCR typically requires extraction and purification of nuclear material. Depending on the sample source, this may involve mechanical manipulation via homogenization or require the use of resins or beads to help bind the nucleic acids prior to purification. Prepare your samples with the help of DNA and RNA extraction, isolation, and purification kits, plus reagents, buffers, and more.

Top Categories

DNA Extraction and Purification

RNA Preparation and Purification

Nucleic Acid Purification Systems

Resources

Boost_Your_DNA_Analysis

Nome file: 22738_BOOST_Your_DNA_Analysis.pdf
Dimensione: 544 KB
Formato: application/pdf

Benchtop_Instrument_Portfolio

Nome file: 22738_Benchtop_Instrument_Portfolio.pdf
Dimensione: 2 MB
Formato: application/pdf

Pall_AcroPrep_Filter_Plates

Nome file: 22738_Pall_AcroPrep_Filter_Plates.pdf
Dimensione: 13 MB
Formato: application/pdf

Pall_Centrifugal_Devices

Nome file: 22738_Pall_Centrifugal_Devices.pdf
Dimensione: 3 MB
Formato: application/pdf

Thermo_Scientific_Guanidinium_Thiocyanate

Nome file: 22738_Thermo_Scientific_Guanidinium_Thiocyanate.pdf
Dimensione: 2 MB
Formato: application/pdf

Thermo_Scientific_Medifuge_Centrifuge

Nome file: 22738_Thermo_Scientific_Medifuge_Centrifuge.pdf
Dimensione: 947 KB
Formato: application/pdf

2. PCR Amplification

Amplification is performed by combining a DNA segment, polymerase, nucleotide solution, and primers in a PCR tube or microplate. A thermal cycler is used to perform the following steps, repeating the process 35 to 40 times.

Denaturation: Hydrogen bonds are broken, DNA separates.

Annealing: Primers and polymerase bind to the DNA.

Extension: Nucleotides pair with the DNA to form a new complementary strand.

Top Categories

Thermal Cyclers

DNA Polymerases

PCR Supplies

Resources

Brand PCR Products

Nome file: Bran_PCR_products.pdf
Dimensione: 4 MB
Formato: application/pdf

PCR Innovations Made to Empower

Nome file: PCR_Innovations_Made_to_Empower.pdf
Dimensione: 2 MB
Formato: application/pdf

Real Time PCR Handbook

Nome file: RT_PCR_Handbook.pdf
Dimensione: 1 MB
Formato: application/pdf

3. qPCR Reverse Transcription

In real-time PCR, RNA is converted to cDNA and then amplified using PCR. Common real-time PCR applications detect expressed genes, examine transcript variants, and create cDNA templates for cloning and sequencing procedures. Find polymerases, oligonucleotides, master mixes, and more below to support your real-time PCR assays.

Top Categories

Real Time PCR Reagents and Kits

DNA Polymerases

Direct PCR Reagents and Kits

Resources

TaqMan Environmental Master Mix 2.0

Nome file: TaqMan_Environmental_Master_Mix_2.0.pdf
Dimensione: 474 KB
Formato: application/pdf

4. qPCR Quantification

Real-time PCR is used to detect specific DNA sequences. This method uses fluorescent dyes or probes to determine the amount of amplified material in your sample. Find a variety of master mixes, plus real-time PCR instruments, assays, and kits for quantifying your qPCR results.

Top Categories

Real Time PCR Master Mixes

qPCR Instruments

Real-Time PCR Assays

Featured Products

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Resources

QuantStudio Absolute Q Digital

Nome file: QuantStudio Absolute Q Digital PCR System.pdf
Dimensione: 1 MB
Formato: application/pdf

QuantStudio Absolute Q Digital PCR System

Nome file: QuantStudio Absolute Q Digital.pdf
Dimensione: 193 KB
Formato: application/pdf

5. Essential Supplies

Find HEPA-filtered PCR enclosures to help keep contaminating particulates out of your workspace. Shop single-use PCR tubes and tube strips, nucleic acid microplates, PCR enclosures, and other labware that is certified free of DNAse, RNAse, DNA, and other potential contaminants.