DiscoverX Ready-to-Assay™ A2A Adenosine Receptor Frozen Cells

The Ready-to-Assay™ A2A Adenosine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. adenosine, NECA) based activation of ADORA2A GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress ADORA2A alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of ADORA2A , the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the cells in a convenient thaw-and-use format.