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Thermo Scientific™ Aminoallyl-dUTP Solution (10 mM)

Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.

Marca:  Thermo Scientific™ R0091

Dettagli aggiuntivi : Peso : 0.00500kg

Codice prodotto. 10510871

  • 121.00€ / 0.10mL
Spedizione stimata: 12-07-2024
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Descrizione

Descrizione

Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.

Aminoallyl-dUTP can be enzymatically incorporated into DNA with:
• Reverse transcriptases
Taq DNA polymerase (Cat. No. EP0072)
• phi29 DNA Polymerase (EP0091)
• Klenow Fragment (EP0051)
• Klenow Fragment, exo- (EP0421)
• DNA Polymerase I (EP0041)

The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin, or hapten.

Applications
• Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis
• PCR
• Nick-translation
• Random-primed labeling
• Primer extension

Specifica

Specifica

Aminoallyl-dUTP Solution, 10 mM (100 μL)

Store at –20°C.
DNA Polymerase
Aminoallyl
Dry Ice
10 mM
Liquid
Direct Labeling
100 μL
cDNA Synthesis, Non-Radioactive Labeling
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For Research Use Only. Not for use in diagnostic procedures.