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Descrizione
Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.
Aminoallyl-dUTP can be enzymatically incorporated into DNA with:
• Reverse transcriptases
• Taq DNA polymerase (Cat. No. EP0072)
• phi29 DNA Polymerase (EP0091)
• Klenow Fragment (EP0051)
• Klenow Fragment, exo- (EP0421)
• DNA Polymerase I (EP0041)
The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin, or hapten.
Applications
• Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis
• PCR
• Nick-translation
• Random-primed labeling
• Primer extension
Specifica
Specifica
| Concentrazione | 10 mM |
| Content And Storage | Aminoallyl-dUTP Solution, 10 mM (100 μL) Store at –20°C. |
| Forma | Liquid |
| Polimerasi | DNA Polymerase |
| Labeling Method | Direct Labeling |
| Label or Dye | Aminoallyl |
| Quantità | 100 μL |
| Shipping Condition | Dry Ice |
| Da utilizzare con (applicazione) | cDNA Synthesis, Non-Radioactive Labeling |
For Research Use Only. Not for use in diagnostic procedures.
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