BD Horizon™ Fixable Viability Stain 520

• Reacts with and covalently binds to cell-surface and intracellular amines
• Permeable plasma cell membranes, such as those present in necrotic cells, allow intracellular diffusion of dye and covalent binding to higher overall concentrations of amines than in non-permeable live cells
• Necrotic cells present in a typical in vitro assay label with higher levels of dye increases their fluorescence intensity 10-20 fold over viable cells
• Labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence
• Excited by Blue laser (with excitation maximum of 498nm) and has fluorescence emission maximum of 521nm

Flow cytometry, Intracellular staining