Invitrogen™ Collibri™ DNA Library Preparation Kit Modules

The Invitrogen Collibri DNA Library Preparation Kit Modules are designed for a custom build of next-generation sequencing (NGS) workflow on Illumina systems. The kit supports whole-genome sequencing (WGS) applications and is suitable for all genomes.

The Collibri DNA Library Preparation Kit Modules support:

• The need for enzymatic manipulation of DNA into a pool of cDNA fragments
• The need to cleanup and size select library to get rid of junk sequences and purify a sample
• The addition of indexed adapters to enable the prepared library sequencing on Illumina instruments

The enzymatic module of the kit generates a consistently high yield of a library, which is enough for library quantification, sequencing, and multiple runs on Illumina instruments.

Enzymatic Shearing (ES) modules

• The Collibri ES DNA Library Preparation Kit Modules are designed for fast and convenient library preparation from a wide range of inputs (1–500 ng), starting from intact genomic DNA or already prepared amplicons (>300 bp) of any species. The entire library preparation protocol takes about three hours with a PCR workflow.
• The Collibri PCR-free ES DNA Library Preparation Kit Modules are designed for fast and convenient library preparation from inputs as low as 100 ng intact genomic DNA or already prepared amplicons (>300 bp) of any species. The entire library preparation protocol takes about two hours for the PCR-free workflow.

Physical Shearing (PS) modules

• The Collibri PS DNA Library Preparation Kit Modules offer library preparation of a wide range of inputs (1 ng to 1 μg) of physically sheared double-stranded DNA, cfDNA, or amplicons (≤300 bp) of any species. The entire library preparation protocol takes about three hours with a PCR workflow. Additionally, a PCR-free version is available for inputs starting at 500 ng DNA.
• The Collibri PCR-free PS DNA Library Preparation Kit modules offer library preparation from inputs as low as 500 ng of physically sheared double-stranded DNA, cfDNA, or amplicons (≤300 bp) of any species. The entire library preparation protocol takes about three hours with a PCR workflow. Additionally, a PCR-free version is available for inputs starting at 500 ng DNA.

If needed, Collibri Library Amplification Master Mix with primers can be bought separately. Or, choose the Collibri ES or PS DNA Library Preparation Kit Modules where these come included with the kit.

Collibri DNA Library Cleanup Kit contains exceptional beads optimized for work with DNA samples. Therefore, the DNA Library Preparation Kit works the best with these cleanup modules and is not recommended to be replaced with a regular bead.

Collibri DNA Indexed adapters contain the highest-quality Combinatorial Dual (CD), or Unique Dual (UD). For the higher throughput use UD and CD together to support up to 192 preparations per run. To avoid overlap of sequences, do not pool together different sizes of modules containing the same type of indexed adaptors (CDs or UDs only).