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Cyclin B1 Antibody (CCNB1/1098), Alexa Fluor™ 594, Novus Biologicals™
Mouse Monoclonal Antibody
Marca: Novus Biologicals NBP2-47893AF594
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Descrizione
Cyclin B1 Monoclonal specifically detects Cyclin B1 in Human, Mouse samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin, Immunofluorescence.
Specifica
| Cyclin B1 | |
| Monoclonal | |
| Alexa Fluor 594 | |
| 50 mM sodium borate with 0.05% sodium azide | |
| CCNB1 | |
| Recombinant human full-length Cyclin B1 protein (Uniprot: P14635) | |
| 0.1 mL | |
| Primary | |
| It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. | |
| Store at 4°C in the dark. |
| Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunofluorescence | |
| CCNB1/1098 | |
| Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunofluorescence | |
| CCNB, cyclin B1, G2/mitotic-specific cyclin B1, G2/mitotic-specific cyclin-B1 | |
| Mouse | |
| Protein A or G purified | |
| Adaptive Immunity, B Cell Development and Differentiation Markers, Cancer, Cell Biology, Cell Cycle and Replication, Cytokine Research, Immunology, Mesenchymal Stem Cell Markers, Mitotic Regulators, Myeloid derived Suppressor Cell, Phospho Specific, Signal Transduction, Stem Cell Lines, Stem Cell Markers, Tumor Suppressors | |
| 891.0 | |
| Human, Mouse | |
| IgG1 κ |
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