Thermo Scientific™ DyLight™ 510-LS NHS Ester

Highlights:

• Single excitation wavelength–DyLight Long Stokes Shift Dyes all can be excited together at 488nm using an Argon laser

• Image multiple targets simultaneously–the four Long Stokes Shift Dyes can be used with DyLight 488 for multiplex fluorescence applications

• NHS ester reactive group–allows immediate labeling of antibodies, proteins, peptides and other amine-containing molecules through amide bond formation

• Multiple solubility options –choose from hydrophilic to hydrophobic dyes to optimize the right dye label for the best performance in an application

• Large Stokes shift inhibits quenching–minimal overlap between excitation and emission peaks inhibits dye-dye quenching effects

• Use in FRET-based assays– the extremely broad separation between excitation and emission peaks allow for use of the Long Stokes Shift Dyes in energy transfer applications where the acceptor dye is spectrally far removed from the excitation wavelength

Criteria to consider when choosing a DyLight Long Stokes Shift Specialty Dye:

• Excitation and emission wavelengths–all dyes share a common excitation wavelength range with options for emission from the green to the far red wavelengths

• Water solubility–DyLight Long Stokes Shift Dyes can be initially solubilized in methanol, ethanol, DMF or DMSO with transfer into an aqueous buffer medium for labeling biomolecules

Recommended for:

Quenchers and FRET pairs; Conjugation to silica particles; STED microscopy; Multicolor microscopy; 4Pi microscopy; Dual-color 3D nanoscopy; Dynamic light scattering; Graded stokes shift; Duplex enhanced RT-PCR; High-resolution optical microscopy