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IBA Lifesciences pASG-IBA E. coli Expression Vector with No Secretion
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Quantità:
5 μg
Dimensione della confezione:
5µg
Descrizione
- The pASG-IBA vectors are designed for expression of target proteins without affinity tag in E. coli.
- The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins.
- The vectors can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli.
- Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity.
Specifica
Specifica
| Agente inducente | Inducible with anhydrotetracycline |
| Gene marcatore | Ampicillin |
| Promotore | Tet |
| Product Type | Vector |
| Content And Storage | Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage |
| Protein Tag | FLAG-tag |
| Quantità | 5 μg |
| Vettore | pASG-IBA63 |
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