Thermo Scientific™ Endonuclease IV, E.coli (2 U/μL)

Cut oxidative damaged DNA at AP sites with this enzyme, which recognizes apurinic/apyrimidinic sites and cleaves the phosphodiester bond 5' to the lesion.

Endonuclease IV (Endo IV) recognizes apurinic/apyrimidinic (AP) sites of dsDNA and cleaves the phosphodiester bond 5' to the lesion generating a hydroxyl group at the 3'-terminus. The enzyme can also act as a 3'-diesterase that is able to release 3'-phosphoglycolate or 3'-phosphate from the damaged ends of dsDNA . Endo IV possesses also a 3'≥5' exonuclease activity. Its progression on substrates is sensitive to ionic strength, metal ions, EDTA, and reducing conditions. Substrates with 3'-recessed ends are preferred substrates for the 3'≥5' exonuclease activity .

The enzyme has no requirement for Mg²⁺ and is fully active in the presence of EDTA in moderate concentrations.