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Descrizione
Thermo Scientific dNTP Set is a convenient set of 100 mM aqueous solutions of each of dATP, dCTP, dGTP and dTTP, supplied in separate vials. These solutions of extremely stable nucleotides are titrated to pH 7.3-7.5 with NaOH. The nucleotides have greater than 99% purity, are free of nuclease activities, human and E. coli DNA. They are designed for many different molecular biology applications.
Features
• Purity confirmed by HPLC
• Free of human and E. coli DNA
Applications
For use in a wide variety of molecular biology applications, including:
• High fidelity and Long range PCR (40 kb)
• PCR, qPCR, RT-PCR, and cDNA synthesis
• RDA, MDA, LAMP-PCR
• DNA labeling and sequencing
Stablity
• Stable after multiple freeze-thaw cycles
• Up to 95% of dNTPs remain in triphosphate form after 7 weeks at room temperature
• Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 min at 94°C, 3 min at 72°C)
Specifica
Specifica
| Concentrazione | 100 mM |
| Content And Storage | dNTP Set, 100 mM solutions (4 x 0.25 mL) Store at –20°C. |
| Forma | Liquid |
| Label or Dye | Unlabeled |
| Purity or Quality Grade | >99% |
| Quantità | 4 x 0.25 ml (4 x 25 µmol di soluzione a 100 mM) |
| Shipping Condition | Dry Ice |
| Da utilizzare con (applicazione) | Standard PCR, High-fidelity PCR, Real Time PCR (qPCR) |
For Research Use Only. Not for use in diagnostic procedures.
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