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Thermo Scientific™ Eam1104I (EarI) (10 U/μL)
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Lambda DNA digested with Eam1104I (EarI), 1% agarose, 34 cleavage sites
conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
5'...C T C T T C (N)1▵...3'
3'...G A G A A G (N)4▵...5'
Conditions for 100% Activity:
- 1X Buffer Tango™:
- 33mM Tris-acetate (pH 7.9 at 37 C), 10 mM Mg-acetate, 66 mM K-acetate and 0.1mg/mL BSA
- Incubate at 37°C
Storage Buffer:
- Eam1104I is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol
Ligation and Recleavage:
- After 50-fold overdigestion with Eam1104I, more than 95% of the DNA fragments can be ligated and recut
Methylation Effects:
- Dam: never overlaps — no effect
- Dcm: never overlaps — no effect
- CpG: may overlap — no effect
- EcoKI: never overlaps — no effect
Digestion of Agarose-embedded DNA:
- Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours
Note:
Certain sites in lambda DNA are difficult to cleave with Eam1104I, the same as with its prototype Ksp632I.
Specifica
Specifica
| Concentrazione | 10 U/μL |
| Methylation Sensitivity | Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive |
| Enzyme | Eam1104I (EarI) |
| Compatible Buffer | 10X Buffer Tango |
| Sensitive to Heat Inactivation | Yes |
| Optimal Reaction Temperature | 37°C |
| Type IIS RE | Yes |
| Quantità | 300 units |
| Product Type | Restriction Enzyme |
| Research Category | Scarless Cloning |
For Research Use Only. Not for use in diagnostic procedures.
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