missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ FastDigest™ HindIII

The FastDigest HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

52.75€ - 142.00€

Specifica

Methylation Sensitivity Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Enzyme Hind III
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Visualizza altre specifiche

Prodotti 2
Codice del prodotto Marca Quantità Prezzo Quantità e disponibilità  
Codice del prodotto Marca Quantità Prezzo Quantità e disponibilità  
10101380
Vedere i documenti Dettagli della promozione
Thermo Scientific™
FD0504
800 µl
52.75€
800 reazioni
Visualizzare prodotti alternativi
Risparmia fino a 
»
In stock
per visualizzare lo stock disponibile
Effettua il per acquistare questo articolo Hai bisogno di un conto web ? Registrati oggi stesso!
11893833
Vedere i documenti Dettagli della promozione
Thermo Scientific™
FD0505
5 x 500 μL (2,500 Reactions)
142.00€
2500 reazioni
Visualizzare prodotti alternativi
Risparmia fino a 
»
In stock
per visualizzare lo stock disponibile
Effettua il per acquistare questo articolo Hai bisogno di un conto web ? Registrati oggi stesso!
Descrizione

Descrizione

Lambda DNA digested with HindIII, 0.7% agarose, 6 cleavage sites

FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Thermo Scientific FastDigest HindIII restriction enzyme recognizes A^AGCTT site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.

Thermo Scientific FastDigest HindIII is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment (Cat. No. EP0051), T4 DNA Ligase (Cat. No. EL0014), alkaline phosphatases and T4 DNA Polymerase (Cat. No. EP0061) all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features

• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.

Specifica

Specifica

Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
10X FastDigest Buffer/FastDigest Green Buffer
37°C
Restriction Enzyme
FastDigest™
80°C
Hind III
Yes
No
Traditional Cloning
5' protruding ends
Videos
SDS
Documenti

Documenti

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.