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Thermo Scientific™ Kit GeneJET™ Plasmid Miniprep

Codice prodotto. 10319699 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
250 Preps
50 Preps
Dimensione della confezione:
250 reazioni
75mL
Pezzo
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Codice prodotto. 10319699

Marca: Thermo Scientific™ K0502

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Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column.

Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure.

Highlights

  • Efficient—high yields of up to 20 μg of high quality plasmid DNA
  • Fast—procedure takes less than 14 minutes
  • Convenient—no phenol/chloroform extraction or alcohol precipitation required
  • Pure—purified DNA is immediately ready to use

Applications

Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:

  • FastDigest or conventional restriction digestion
  • Automated fluorescent and radioactive sequencing
  • PCR
  • In vitro transcription
  • Transformation

Principle

A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing.

Components

  • Resuspension Solution
  • Lysis Solution
  • Neutralization Solution Wash Solution (concentrated)
  • RNase A Solution
  • Elution Buffer
  • Spin Columns
  • Collection Tubes
  • Detailed Protocol

Related Products

  • RNase A (Part No.: R1253)
  • Elution Buffer for GeneJET Plasmid Miniprep Kit (Part No.: R1263)
  • Lysis Solution for GeneJET Plasmid Miniprep Kit (Part No.: R1223)
  • Resuspension Solution for GeneJET Plasmid Miniprep Kit (Part No.: R1213)
  • Neutralization Solution for GeneJET Plasmid Miniprep Kit (Part No.: R1233)
  • Wash Solution for GeneJET Plasmid Miniprep Kit (Part No.: R1243)
  • Binding Buffer for GeneJET Plasmid Miniprep Kit(Part No.: R1331)
TRUSTED_SUSTAINABILITY

Specifica

Tipo di colonna Spin Column
Formato Spin Column
Isolation Technology Silica Column
Bilancia Mini
Tipo di campione Bacteria
Da utilizzare con (applicazione) PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription
Compatibilità ad alta produttività Compatible (Vacuum manifold)
Prep Scale < 100 μg (Small-Scale) Plasmid DNA
Linea di prodotti GeneJET
Product Type Plasmid MiniPrep Kit
Quantità 50 Preps
Bersaglio Plasmid DNA
Durata del test <14 min
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compliance-icons
Product Identifier
  • GeneJET Plasmid Miniprep Kit
Signal Word
  • Attenzione
Hazard Category
  • Tossicità acuta Categoria 4
  • Lesione oculare grave/irritazione oculare Categoria 2
  • Corrosione/irritazione della pelle Categoria 2
Hazard Statement
  • H302-Nocivo se ingerito.
  • H315-Provoca irritazione cutanea.
  • H319-Provoca grave irritazione oculare.
Precautionary Statement
  • P264-Lavare accuratamente dopo l'uso.
  • P270-Non mangiare, né bere, né fumare durante l'uso.
  • P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
  • P301+P310-IN CASO DI INGESTIONE: contattare immediatamente un CENTRO ANTIVELENI/un medico/
  • P302+P352-IN CASO DI CONTATTO CON LA PELLE: lavare abbondantemente con acqua/.
  • P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
  • P330-Sciacquare la bocca.
  • P332+P313-In caso di irritazione della pelle: consultare un medico.
  • P337+P313-Se l'irritazione degli occhi persiste, consultare un medico.
  • P362+P364-Togliere tutti gli indumenti contaminati e lavarli prima di indossarli nuovamente.
  • P501b-Smaltire il prodotto/recipiente in conformità con le disposizioni locali/regionali/nazionali/internazionali.
Supplemental information
  • MIXTURE LIST-Contiene: guanidine hydrochloride

For Research Use Only. Not for use in diagnostic procedures.

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