Thermo Scientific™ Kit GeneJET™ Plasmid Miniprep

Highlights

• Efficient—high yields of up to 20 μg of high quality plasmid DNA
• Fast—procedure takes less than 14 minutes
• Convenient—no phenol/chloroform extraction or alcohol precipitation required
• Pure—purified DNA is immediately ready to use

Applications

Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:

• FastDigest or conventional restriction digestion
• Automated fluorescent and radioactive sequencing
• PCR
• In vitro transcription
• Transformation

Principle

A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing.

Components

• Resuspension Solution
• Lysis Solution
• Neutralization Solution Wash Solution (concentrated)
• RNase A Solution
• Elution Buffer
• Spin Columns
• Collection Tubes
• Detailed Protocol

Related Products

• RNase A (Part No.: R1253)
• Elution Buffer for GeneJET Plasmid Miniprep Kit (Part No.: R1263)
• Lysis Solution for GeneJET Plasmid Miniprep Kit (Part No.: R1223)
• Resuspension Solution for GeneJET Plasmid Miniprep Kit (Part No.: R1213)
• Neutralization Solution for GeneJET Plasmid Miniprep Kit (Part No.: R1233)
• Wash Solution for GeneJET Plasmid Miniprep Kit (Part No.: R1243)
• Binding Buffer for GeneJET Plasmid Miniprep Kit(Part No.: R1331)