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  7. Thermo Scientific™ Maxima Probe/ROX qPCR Master Mix (2X)

Thermo Scientific™ Maxima Probe/ROX qPCR Master Mix (2X)

Codice prodotto. 11803923 Sfoglia Tutto Thermo Scientific Prodotti
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N. di reazioni:
1,000 Reactions
200 reactions
4,000 Reactions
Dimensione della confezione:
1000 reazioni
200 reazioni
4000 reazioni
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Codice prodotto. 11803923

Marca: Thermo Scientific™ K0231

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Description

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.

Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.

The master mix includes Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. It is supplemented with ROX passive reference dye. Only template and primers need to be added.Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carryover contamination control using uracil DNA glycosylase (UDG). ROX serves as a passive reference dye. The use of Maxima Probe/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. The master mix can be used with most real-time thermal cyclers, including instruments from Applied Biosystems, Eppendorf, Corbett, Bio-Rad and Roche.
  • Sensitivity—detects low copy number targets
  • Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Wide linear range—accurate quantification across 9 orders of magnitude
  • Universal—can be used with sequence-specific probes on most real-time thermal cyclers
  • Reproducibility and convenience—ready-to-use 2X master mix minimizes pipetting error and reduces set-up time

Recommended for:

Real-time PCR using sequence-specific probes; Real-time RT-PCR using sequence-specific probes.

TRUSTED_SUSTAINABILITY

Specifications

Concentrazione 2X
Content And Storage Contains:
• 2 x 1.25 mL Maxima Probe/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer with ROX passive reference dye; sufficient for 200 x 25 μL reactions.
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.
Metodo di rilevamento Primer-probe
Formato Tube
GC-Rich PCR Performance High
PCR Method qPCR
Polimerasi Taq DNA Polymerase
Reaction Speed Standard
Da utilizzare con (apparecchiatura) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Linea di prodotti Maxima
Product Type qPCR Master Mix
Quantità 200 reactions
Sufficiente per 200 Reactions of 25 μL
Da utilizzare con (applicazione) Gene Expression
N. di reazioni 200 reactions
Tipo di campione DNA, dsDNA
Volume 2 x 1.25 mL
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For Research Use Only. Not for use in diagnostic procedures.

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