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  7. Thermo Scientific™ Miscela master per qPCR Maxima™ SYBR™ Green 2X con soluzione ROX™

Thermo Scientific™ Miscela master per qPCR Maxima™ SYBR™ Green 2X con soluzione ROX™

Codice prodotto. 11853923 Sfoglia Tutto Thermo Scientific Prodotti
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N. di reazioni:
1000 reazioni da 25 µl
200 reazioni da 25 µl
4,000 Reactions
Dimensione della confezione:
1000 reazioni
200 reazioni
4000 reazioni
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Product Code. 11853923

Brand: Thermo Scientific™ K0251

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Description

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers.

  • Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity - detects low copy number targets
  • Wide linear range - accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience - ready-to-use 2X master mix
TRUSTED_SUSTAINABILITY

Specifications

Concentrazione 2X
Content And Storage Contains:
• 2 x 1.25 mL Maxima SYBR Green qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 200 x 25 μL reactions.
• 50 μL ROX solution, 50 μM
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.
Metodo di rilevamento SYBR
Formato Tube
GC-Rich PCR Performance High
PCR Method qPCR
Polimerasi Taq DNA Polymerase
Reaction Speed Standard
Da utilizzare con (apparecchiatura) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Linea di prodotti Maxima
Product Type Real Time PCR SYBR Master Mix
Quantità 200 reactions
Sufficiente per 200 Reactions of 25 μL
Da utilizzare con (applicazione) Gene Expression
N. di reazioni 200 reazioni da 25 µl
Tipo di campione DNA, dsDNA
Volume 2 x 1.25 mL
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For Research Use Only. Not for use in diagnostic procedures.

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