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Thermo Scientific™ Miscela master per qPCR Maxima™ SYBR™ Green 2X con soluzione ROX™
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The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers.
- Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
- Sensitivity - detects low copy number targets
- Wide linear range - accurate quantification across 9 orders of magnitude
- Reproducibility and convenience - ready-to-use 2X master mix
Specifica
Specifica
| Concentrazione | 2X |
| Content And Storage | Contains: • 10 x 1.25 mL Maxima SYBR Green qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 1000 x 25 μL reactions. • 250 μL ROX solution, 50 μM • 10 x 1.25 mL Nuclease-Free Water Store at -20°C. |
| Metodo di rilevamento | SYBR |
| Formato | Tube |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Polimerasi | Taq DNA Polymerase |
| Reaction Speed | Standard |
| Da utilizzare con (apparecchiatura) | BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene |
| Linea di prodotti | Maxima |
| Vedi altri risultati |
For Research Use Only. Not for use in diagnostic procedures.
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