Thermo Scientific™ MboII (5 U/μL)

5'...G A A G A (N)₈▵...3'

3'...C T T C T (N)₇▵...5'

Conditions for 100% Activity:

• 1X Buffer B: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl₂ and 0.1mg/mL BSA
• Incubate at 37°C

Storage Buffer:

• MboII is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

• Dam: may overlap —blocked
• Dcm: never overlaps —no effect
• CpG: may overlap —no effect
• EcoKI: never overlaps —no effect
• EcoBI: may overlap —no effect

Note:

Assayed using lambda DNA (dam) (#SD0021). MboII is blocked by overlappingdam methylation. To avoiddam methylation, use adam,dcm- strain such as GM2163 (#M0099). Greater than 15-fold overdigestion with MboII may result in star activity. MboII produces DNA fragments that have a single-base 3 extension which are more difficult to ligate than blunt-ended fragments. MboII may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.