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Thermo Scientific™ T3 RNA Polymerase
Thermo Scientific™ Fermentas T3 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.
Marca: Thermo Scientific™ EP0102
Dettagli aggiuntivi : Peso : 0.01000kg
Descrizione
Thermo Scientific™ Fermentas Bacteriophage T3 RNA polymerase is DNA-dependent RNA polymerase with strict specificity for their respective double-stranded promoters.
It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from its promoter.- Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)
Recommended for:
Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.
Note:
Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA–T3 TAATACGACTCACTATA G GGAGA–T7 ATTTAGGTGACACTATA G AAGNG–SP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).
Specifica
| 20U/µL | |
| Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing. | |
| 10,000 U |
| T3 RNA Polymerase | |
| 1mL of 5X Transcription Buffer |