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Fisher BioReagents™ Taq DNA Polymerase

Codice prodotto. 10104974
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Composizione:
10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial
Quantità:
5 × 2500 Units
Dimensione della confezione:
pezzi 5
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Codice prodotto. Composizione Quantità unitSize
10104974 10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial 5 × 2500 Units pezzi 5
10458923 - - -
10448733 - - -
10717185 - - -
10091263 - - -
10458733 - - -
10698393 - - -
10144924 10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial 5 × 2500 Units pezzi 5
10449693 - - -
10346363 - - -
10216744 - - -
10356363 - - -
10206744 - - -
10071553 - - -
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Codice prodotto. 10104974 Fornitore Fisher BioReagents™ N. del fornitore FB600075
 Articolo soggetto a restrizioni
Spedizione stimata: 23-11-2026
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Descrizione

Allows up to 100 cycles of amplification without loss of activity

  • Licensed for use in PCR
  • Uses a thermostable DNA polymerase that has been encoded by a modified form of Thermus aquaticus DNA polymerase gene that has been inserted into an E. coli host
  • Has a 5«→3« exonuclease activity
  • Available with two different reaction buffers
  • Buffer A contains an optimized final concentration of 15mM MgCl2 for most PCR amplifications
  • Buffer B does not contain MgCl2; MgCl2 is provided in a separate vial as a 25mM solution
  • Storage Buffer: 20mM Tris-HCl, pH 7.5 (at room temperature), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% Tween™ 20, 0.5% Nonidet P40™
  • Recommended Assay Buffer: 10X Assay Buffer A (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl; 15mM MgCl2) or 10X Assay Buffer B (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl) and 25mM MgCl2 solution in a separate vial

Unit Definition: One unit of enzyme is defined as the amount that will incorporate 10nmol of dNTPs into acid-insoluble material per 30 minutes in a 10-minute incubation at 74°C (165°F) under the assay conditions above

TRUSTED_SUSTAINABILITY

Especificaciones

Concentrazione 5 U/μL
Composizione 10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial
Content And Storage -20°C
Descrizione Taq DNA Polymerase
Forma Liquid
Formato Poly Tube
Quantità 5 × 2500 Units
Da utilizzare con (applicazione) Standard PCR
Tampone di conservazione Tris-HCl 20mM, pH 7.5 (at room temperature), KCl 100mM, EDTA 0.1mM, DTT 1mM, Glycerol 50%, Tween™ 20 0.5%, Nonidet™ P40™ 0.5%
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