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Fisher BioReagents™ Taq DNA Polymerase

Codice prodotto. 10104974
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Composizione:
10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial
Quantità:
100 Units
25 × 100 Units
2500 Units
5 × 100 Units
5 × 2500 Units
5 × 500 Units
500 Units
Dimensione della confezione:
100 unità
2500 unità
500 unità
pezzi 5
14 product options available for selection
Product selection table with 14 available options. Use arrow keys to navigate and Enter or Space to select.
Codice prodotto. Composizione Quantità unitSize
10104974 10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial 5 × 2500 Units pezzi 5
10458923 10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial 5 × 500 Units 2500 unità
10448733 10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial 100 Units 100 unità
10717185 10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial 25 × 100 Units 2500 unità
10091263 10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial 5 × 100 Units 500 unità
10458733 10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial 500 Units 500 unità
10698393 10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial 2500 Units 2500 unità
10144924 10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial 5 × 2500 Units pezzi 5
10449693 10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial 100 Units 100 unità
10346363 10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial 5 × 100 Units 500 unità
10216744 10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial 2500 Units 2500 unità
10356363 10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial 500 Units 500 unità
10206744 10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial 25 × 100 Units 2500 unità
10071553 10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial 5 × 500 Units 2500 unità
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 14 options available.
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Codice prodotto. 10104974 Fornitore Fisher BioReagents™ N. del fornitore FB600075
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Descrizione

Allows up to 100 cycles of amplification without loss of activity

  • Licensed for use in PCR
  • Uses a thermostable DNA polymerase that has been encoded by a modified form of Thermus aquaticus DNA polymerase gene that has been inserted into an E. coli host
  • Has a 5«→3« exonuclease activity
  • Available with two different reaction buffers
  • Buffer A contains an optimized final concentration of 15mM MgCl2 for most PCR amplifications
  • Buffer B does not contain MgCl2; MgCl2 is provided in a separate vial as a 25mM solution
  • Storage Buffer: 20mM Tris-HCl, pH 7.5 (at room temperature), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% Tween™ 20, 0.5% Nonidet P40™
  • Recommended Assay Buffer: 10X Assay Buffer A (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl; 15mM MgCl2) or 10X Assay Buffer B (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl) and 25mM MgCl2 solution in a separate vial

Unit Definition: One unit of enzyme is defined as the amount that will incorporate 10nmol of dNTPs into acid-insoluble material per 30 minutes in a 10-minute incubation at 74°C (165°F) under the assay conditions above

TRUSTED_SUSTAINABILITY

Specifica

Concentrazione 5 U/μL
Composizione 10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial
Content And Storage -20°C
Descrizione Taq DNA Polymerase
Forma Liquid
Formato Poly Tube
Quantità 5 × 2500 Units
Da utilizzare con (applicazione) Standard PCR
Tampone di conservazione Tris-HCl 20mM, pH 7.5 (at room temperature), KCl 100mM, EDTA 0.1mM, DTT 1mM, Glycerol 50%, Tween™ 20 0.5%, Nonidet™ P40™ 0.5%
Titolo del prodotto
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