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Fisher BioReagents™ Optizyme™ Ribonuclease Inhibitor, Recombinant
Protects RNA from degradation by RNases
Marca: Fisher BioReagents™ BP3222-1
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Descrizione
- Supplied as a recombinant product from E. coli (originally isolated from rat lung) and provides superior protection of RNA from degradation by RNases
- Commonly added to all solutions used during the isolation of RNA, and will not interfere with the performance of most enzymes in downstream applications
- Active over a broad temperature range, and even provides some RNase inhibition at 60°C, which is useful when performing reverse transcription reactions at elevated temperatures to overcome secondary structure in RNA
- Storage Buffer Components: 20mM HEPES-KOH (pH 7.6 at 4°C), 50mM KCl, 8mM DTT, and 50% glycerol (v/v)
Inactivates RNase A, RNase B, and RNase T2; No activity against SP6, T7, or T3 RNA polymerases, AMV or MMLV reverse transcriptases, and Taq DNA polymerase
- Displays a broad spectrum of inhibitory activity against RNases, and does not have activity against other polymerases and reverse transcriptases
- Suitable for use in common molecular biology applications such as isolation and purification of RNA, cDNA synthesis, RT-PCR, in vitro RNA transcription/translation, ribonuclease protection assay, and preparation of RNase-free antibodies
Specifica
| 20 to 40 U/μL | |
| Isolation and purification of RNA, cDNA synthesis, RT-PCR, in-vitro RNA transcription/translation, Ribonuclease protection assay and preparation of RNase-free Antibodies | |
| E. coli | |
| RNase Inhibitor |
| Colorless | |
| 20mM HEPES-KOH (pH 7.6 at 4°C), 50mM KCl, 8mM DTT, 50% Glycerol | |
| 2500 U | |
| Liquid |