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Invitrogen™ GeneArt™ CRISPR Nuclease mRNA

Codice prodotto. 15411054
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Quantità:
15 μg
Dimensione della confezione:
15µg
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Codice prodotto. Quantità Dimensione della confezione
15411054 15 μg 15µg
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Codice prodotto. 15411054 Fornitore Invitrogen™ N. del fornitore A29378
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Descrizione

Invitrogen™ GeneArt™ CRISPR Nuclease mRNA is formulated using a new manufacturing process to achieve superior quality and improved product performance.

  • This wild-type Cas9 mRNA has two nuclear localization signals (NLSs).
  • Cas9 constructs with two NLSs have been shown to be more efficient at targeting the Cas9 protein to the nucleus (1).
  • The mRNA format is ready to transfect and circumvents the need for time-consuming cloning steps required when using CRISPR vector systems.
  • Cas9 mRNA is co-transfected with a target-specific guide RNA (gRNA) to direct the Cas9 protein to the intended genome locus to create a double-stranded break.
  • The complete RNA format is a smaller payload than plasmid-based Cas9 systems for improved delivery into the cell and better genome editing efficiency.
  • Additionally, the Cas9 mRNA can be used in multiplex approaches with more than one gRNA.
  • Use this approach to determine which gRNA sequence works best for a particular target or to edit multiple genomic loci with one transfection.
TRUSTED_SUSTAINABILITY

Specifica

Content And Storage 1 vial CRISPR Nuclease mRNA. Store in ultra-cold freezer -68 to -85°C
Quantità 15 μg
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