Proteintech™ Human Galectin-3 ELISA Kit

• Recognizes human Galectin-3
• Galectins are a family of animal lectins defined by shared characteristic amino acid sequences and affinity for β-galactose-containing oligosaccharides
  • Galectin-3 is a 31 kDa member of β-galactoside-binding proteins with one carbohydrate recognition domain and a proline and glycine rich N-terminal domain through which is able to form oligomers
  • Widely expressed in many normal tissues and tumors
  • Found intracellularly in nucleus and cytoplasm or secreted outside of cell, present on cell surface or in extracellular space
  • Involved in biological processes (cell growth, adhesion, differentiation, apopotosis, angiogenesis, immune response, neoplastic transformation, metastasis)
  • Elevated serum Galectin-3 levels reported in patients with breast, gastrointestinal, lung or ovarian cancer, melanoma and non-Hodgkin's lymphoma

Process:

• Antibody specific for human Galectin-3 pre-coated onto microwells
• Human Galectin-3 protein in samples captured by the coated antibody after incubation
• After extensive washing, another antibody of biotinylated specific for human Galectin-3 is added to detect captured human Galectin-3 protein
• For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent
• Solution containing sulfuric acid used to stop color development and color intensity
  • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm