Proteintech™ Human IL-27 ELISA Kit

• Recognizes human IL27
• IL27 is a member of IL12/IL23 heterodimeric family of cytokines, has pleiotropic properties can enhance or limit immune responses
  • Two subunits IL27a and IL27b
  • IL27 acts on cell types (T cells, B cells, macrophages, dendritic cells, natural killer cells and non-hematopoietic cells)
  • Plays role in early regulation of T helper Type 1 initiation and enhances proliferation of naive CD4+T cells and naive B cells
  • Exerts anti-inflammatory functions by inhibiting development of Th17 cells and inducing IL10 producing Type 1 regulatory T cells
  • Potentially promising cytokine for therapeutic approaches on various diseases

Process:

• Antibody specific for human IL27 pre-coated onto microwells
• Human IL27 protein in samples captured by the coated antibody after incubation
• After extensive washing, another antibody of biotinylated specific for human IL27 added to detect captured human IL27 protein
• For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent
• Solution containing sulfuric acid used to stop color intensity
  • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm