Invitrogen™ Human LAP/TGFb ProQuantum Immunoassay Kit

ELISA

The ProQuantum Human LAP/TGFb Immunoassay Kit is designed to provide quantitative measurements of human LAP/TGFb in small sample volumes. Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive software for analytics enables sample-to-answer in just 2 hours. High sensitivity-detect low levels of protein with greater sensitivity than traditional methods Broad dynamic range-5 logarithmic units, minimizing sample dilutions to ensure they fall within the range Small sample consumption-use 2-5 μL of sample (compared to 75 μL for triplicate wells with other methods) Fast, easy workflow-2 hours from sample to answer, with no wash steps No proprietary instrument to purchase-runs on any real-time PCR instrument ProQuantum immunoassays utilize a matched pair of target-specific antibodies, each conjugated to a DNA oligonucleotide. During antibody-analyte binding, the two DNA oligos are brought into close proximity, which allows for ligation of the two strands and subsequent creation of a template strand for amplification. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology (Figure 1). The assay...