Lysis buffer BQ1, Macherey Nagel Bioanalysis™

Flexible handling of multiples of 8 samples is achieved by use of a reusable column holder. Parallel lysis of large sample numbers can be performed in standard microcentrifuge tubes or in 96 well blocks alternatively.NucleoSpin™ Blood QuickPure binding strips are to be used in a centrifuge with deep buckets allowing processing of NucleoSpin™ 8 well strips stacked on 96 well blocks. For further information contact our Technical Support Team.The method is based on a specially treated silica membrane which speeds up the procedure by a combined washing/drying step. The number of washing and drying steps is reduced from 3 to only 1, therefore hands-on time is minimised.Lysis is achieved by incubation of whole blood in a solution containing large amounts of chaotropic ions in the presence of proteinase K. Appropriate conditions for binding of DNA to the silica membrane of the NucleoSpin™ Blood QuickPure binding strips are created by addition of ethanol to the lysate. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer and can be used directly for PCR*, Southern blotting or any kind of enzymatic reaction. The kit allows isolation of 4μg to 6μg of pure genomic DNA from 200μL whole blood with an A_260/280 ratio between 1.60 and 1.90 and a typical concentration of 40ng/μL to 60ng/μL.Kit components: NucleoSpin™ Blood QuickPure binding strips, buffers, proteinase K, accessories.

• Silica membrane technology
• Manual processing
• Yield: 4μg to 6μg
• Sample material: human or animal whole blood (200μL/300μL†)
• Elution volume: 7.5μL to 100μL
• Binding capacity: 60μg
• Preparation time: 60min/12 strips

Ultra-fast, small-scale purification of genomic DNA from whole blood, serum, plasma or other body fluids.
† For preparation of 300μL samples increased volumes of lysis buffer BQ1 required.