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IBA Lifesciences pCSG-IBA Mammalian Expression Vector with No Secretion
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Codice prodotto. 15673858 Sfoglia Tutto IBA Lifesciences Prodotti
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Vettore:
pCSG-IBA103
pCSG-IBA105
pCSG-IBA123
pCSG-IBA143
pCSG-IBA145
pCSG-IBA162
pCSG-IBA164
pCSG-IBA167
pCSG-IBA168
pCSG-IBA23
pCSG-IBA3
pCSG-IBA43
pCSG-IBA45
pCSG-IBA5
pCSG-IBA62
pCSG-IBA64
pCSG-IBAwt1
Förpackningsstorlek
5µg
Denne vare kan ikke returneres. Se returpolitik

Produktkode 15673858

missing translation for 'mfr': IBA Lifesciences 55043001

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Beskrivelse

Designed for high-level episomal expression in mammalian hosts under G418 selection. IBA Lifesciences GmbH Mammalian Expression Vector with No Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR).

pCSG-IBA mammalian expression vectors

  • Designed for high-level episomal expression in mammalian hosts under G418 selection containing the following elements:
    • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
    • Episomal replication through Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7)
  • Stable cell lines can be selected by the neomycin resistance gene (NeoR).
  • In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells.

Please note that cloning into pCSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry-vector is possible.

Tekniske data

Sistema costitutivo o inducibile Constitutive
Gene marcatore Ampicillin
Promotore CMV
Product Type Vector
Agente di selezione (eucariotico) Geneticin™ (G-418)
Stabilità 12 months after shipping
Content And Storage Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Specie Mammalian
Shipping Condition Room temperature
Protein Tag His Tag (6x), Strep-tag II
Cloning Method Direct Cloning
Concentrazione 250 ng/μL
Primer Forward
Quantità 5 μg
Vettore pCSG-IBA43
Da utilizzare con (applicazione) Episomal expression (oriP, EBNA-1)
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