Thermo Scientific™ Maximum Recovery Diluent

The isotonic strength of Maximum Recovery Diluent ensures recovery of organisms from various sources which may be vulnerable in distilled water or aqueous suspensions.

The low concentration of peptone does not cause multiplication of the organisms within 1 to 2 hours of dilution of the sample.

Technique:

1. Put 10g of the test sample into a sterile blender jar or sterile plastic bag.
2. Add 90mL of sterile Maximum Recovery Diluent.
3. Operate the blender according to its speed for sufficient time to give a total number of 15,000 to 20,000 revolutions. Alternatively, operate a peristaltic type blender (Stomacher) for 2 minutes.
4. Within 15 minutes, transfer 1mL of the macerate to 9mL of sterile diluent and mix well (10-1 dilution).
5. Prepare additional decimal dilutions in the same way.
6. Aseptically transfer 1mL of each dilution of the initial suspension in duplicate to the center of a dish.
7. Prepare pour plates with the medium of choice.
8. Allow the agar to solidify and incubate.

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