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Invitrogen™ Mouse LAP/TGFb ProQuantum Immunoassay Kit
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Quantità:
5 x 96 Tests
96 Tests
Dimensione della confezione:
Pezzo
pezzi 96
Descrizione
Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive software for analytics enables sample-to-answer in just 2 hours.
- High sensitivity-detect low levels of protein with greater sensitivity than traditional methods
- Broad dynamic range-≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range
- Small sample consumption-use 2–5 μL of sample (compared to 75 μL for triplicate wells with other methods)
- Fast, easy workflow-2 hours from sample to answer, with no wash steps
- No proprietary instrument to purchase-runs on any real-time PCR instrument
TGF beta-1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its prodomain. Activation of TGF-b1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF-beta, an aminoterminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP.
Specifica
Specifica
| Numero di accesso | CED; DPD1; LAP; Latency-associated peptide; prepro-transforming growth factor beta-1; regulatory protein; TGFB; TGFB1; Tgfb-1; TGFbeta; TGF-beta 1; TGFbeta1; TGF-beta1; TGF-beta-1; transforming growth factor beta 1; transforming growth factor beta-1; Transforming growth factor beta-1 proprotein; transforming growth factor, beta 1; transforming growth factor-beta 1 |
| Intervallo saggio | 0.032 to 2500 ng/mL |
| Assay Sensitivity | <0.03 ng/mL |
| Coniugato | FAM™ |
| Product Type | ProQuantum |
| Tipo di campione | Plasma, Serum, Supernatant |
| Da utilizzare con (apparecchiatura) | qPCR Instruments |
| ID gene (immissione) | 21803 |
| Simbolo del gene | TGFB1 |
| Contenuto kit | Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase |
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