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Applied Biosystems™ MycoSEQ™ Plus Mycoplasma Detection Kits

Codice prodotto. 18755455 Sfoglia Tutto Thermo Scientific Prodotti
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Include:
Mycoplasma Detection Kit Only
PrepSEQ™ 1-2-3 Nucleic Acid Extraction Kit
PrepSEQ™ Express Nucleic Acid Extraction Kit
Dimensione della confezione:
100 reazioni
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Codice prodotto. Include Pakningsstørrelse
18755455 PrepSEQ™ Express Nucleic Acid Extraction Kit 100 reazioni
18765485 Mycoplasma Detection Kit Only Pezzo
18872368 Mycoplasma Detection Kit Only -
18775505 PrepSEQ™ 1-2-3 Nucleic Acid Extraction Kit Pezzo
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Artikelnummer. 18755455 Leverandør Applied Biosystems™ Leverandørnr. A57926
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    5050.00€
    4870.00€ / 100 reazioni
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Beskrivelse

The MycoSEQ™ Plus Mycoplasma Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for the detection of mycoplasma in complex bioproduction samples.

The MycoSEQ™ Plus Mycoplasma Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for the detection of mycoplasma in complex bioproduction samples. Designed and tested using criteria often applied toward rapid mycoplasma detection in biotherapeutic manufacturing cell culture lot release, the MycoSEQ Plus kit enables results that meet or exceed guidance on sensitivity and specificity expectations as described in European Pharmacopoeia (E.P. 2.6.7, 2007), US Pharmacopoeia (US63) and Japanese Pharmacopoeia. When used with a suitable sample preparation method, the MycoSEQ Plus kit can detect less than 10 CFU/mL or the genomic equivalent of 10 GC/mL.

This multiplexed assay enables predicted simultaneous screening of over 200 species of Mycoplasma target, discriminatory detection of an assay specific control template, and internal PCR control with no cross-reactivity to closely-related bacterial species.

Features of the MycoSEQ Plus Mycoplasma Detection Kit

  • Provides results in less than 1 day
  • Includes a unique discriminatory positive control to differentiate a true positive result from a potential cross-contamination positive call
  • Includes protocols for various methods
  • Live mycoplasma is not needed: MycoSEQ Mycoplasma Detection Kit does not contain any live mycoplasma and does not require use of live mycoplasma for validation
  • Can be used with gDNA, inactivated mycoplasma or live mycoplasma
  • May be used as an alternative to the standard 28-day culture test
  • May be used in conjunction with culture-based methods by providing preliminary results while waiting for results from the 28-day test

PrepSEQ nucleic acid extraction kits offer a robust and easy workflow to overcome complex matrix challenges to ensure the required sensitivity. Use MycoSEQ Plus Mycoplasma Detection Kit with PrepSEQ 1-2-3 Nucleic Acid Extraction Kit (Cat. No. A57925) for manual extractions, or MycoSEQ Plus Mycoplasma Detection Kit with PrepSEQ Express Nucleic Acid Extraction Kit (Cat. No. A57926) for automated extraction using the Automate Express Nucleic Acid Extraction System.

Integrated workflow solution

The MycoSEQ Plus Mycoplasma Detection Kit is part of an integrated workflow for adventitious agent, impurity and contaminant testing during biopharmaceutical manufacturing. The entire workflow including PrepSEQ Sample Preparation Kits, which offer manual or automated sample preparation approaches, together with qPCR analysis can provide results in under 5 hours. (Please note complex matrices may require additional upfront processing steps, as described in various available protocols.)

MycoSEQ Plus kit performance has been tested and confirmed on the Applied Biosystems 7500 Fast Real-Time PCR System and the Applied Biosystems QuantStudio 5 Real-Time PCR System. Data analysis using AccuSEQ Real-Time PCR Detection Software provides accurate quantitation and security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance.

TRUSTED_SUSTAINABILITY

Tekniske data

Content And Storage MycoSEQ Mycoplasma Detection Kit
• 2X qPCR Master Mix Plus, -15 to -25°C
• MycoSEQ Plus 10X Assay Mix, -15 to -25°C
• Negative Control, -15 to -25°C until first thawed, then store at 2°C to 8°C
• DNA Dilution Buffer, -15 to -25°C until first thawed, then store at 2°C to 8°C
• MycoSEQ Plus Discriminatory Positive Control, -15 to -25°C

PrepSEQ Express Nucleic Acid Sample Extraction Kit
• PrepSEQ Express Cartidges, store at 18-25°C
• Lysis Tubes, store at 18-25°C
• Sample Tubes, store at 18-25°C
• Elution Buffer, store at 18-25°C
• Elution Tubes, store at 18-25°C
• AutoMate Exptress Tips and Tip Holders, store at 18-25°C
Descrizione MycoSEQ™ Plus Mycoplasma Detection Kit with PrepSEQ™ Express Nucleic Acid Extraction Kit
Metodo di rilevamento Primer-probe
Forma Liquid
GC-Rich PCR Performance Medium
PCR Method Multiplex qPCR
Reaction Speed Standard
Da utilizzare con (apparecchiatura) QS5 0.1 mL Block or 7500 FAST
Label or Dye FAM
Linea di prodotti MycoSEQ
Product Type Mycoplasma Detection
Quantità 100 Reactions
Shipping Condition Dry Ice
Target Organism Class Mollicutes, mycoplasma
Da utilizzare con (applicazione) Microbial Detection
N. di reazioni 100 Reactions
Tipo di campione Biopharmaceutical Purification Sample, Bulk Drug Substance Sample, Cell Cultures
Include PrepSEQ™ Express Nucleic Acid Extraction Kit
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For Research Use Only. Not for use in diagnostic procedures.

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