Invitrogen™ Luminex™ Intracellular Protein Buffer Reagent Kit

The Intracellular Protein Buffer Reagent Kit is comprised of the reagents intended for use with Human, Mouse and Rat Intracellular Singleplex Bead Kits. Singleplex Bead Kits have been developed to maximize flexibility in experimental design, permitting the measurement of one or multiple analytes in panels designed by the researcher. These reagents are suitable for use with the Luminex 100™/200™ and the FLEXMAP 3D™ systems.

Luminex xMAP™ Technology—Effective and Proven Analytical Tool
The Human, Mouse and Rat Intracellular Singleplex Bead Kits used in conjunction with the Intracellular Protein Buffer Reagent Kit are based on xMAP™ technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex assays. Polystyrene microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead is given a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay, utilizing a 96-well microplate format. At the completion of the sandwich immunoassay, beads can be read using theLuminex 100/200 or FLEXMAP 3D detection systems, instruments based on flow cytometry, two lasers, real-time digital signal processing, and xPONENT™ software to distinguish bead color (analyte) and assay signal strength (PE) fluorescence intensity. The Human, Mouse and Rat Intracellular Singleplex Bead Kits that are used with the Intracellular Protein Buffer Reagent Kit have been validated using the rigorous criterion that distinguishes Luminex assays from other assays.

• Accurate, reproducible, and sensitive quantitation of multiple proteins
• Fully qualified antibodies permit specificity and sensitivity
• Performs assay and analyzes data typically in less than one day

Cell Analysis, Immunoassays, Ready-To-Use Immunoassay