Invitrogen™ Novex TNF- Mouse ELISA Kit

• The total assay incubation time is only 2.5 hour
• A monoclonal capture antibody specific for Mouse TNF-α has been coated onto the wells of the 96-well plate provided
• Samples, including a standard of known Mouse TNF-α content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinylated polyclonal detector antibody
• During the first incubation, Mouse TNF-α antigen binds to the immobilized capture antibody and to the solution phase biotinylated detector antibody on a second site
• After washing, a streptavidin-horseradish peroxidase (HRP) is added
• This binds to the biotinylated detection antibody to complete the four–member sandwich
• After a second incubation and washing to remove all unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color
• The intensity of this colored product is directly proportional to the concentration of Mouse TNF-α present in the original specimen and the optical density can be read on a standard microplate reader

The kit comes with a capture antibody precoated to the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips allowing you to run as few samples as you want. Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency.

Apoptosis, Apoptosis Related Factors, Cell Analysis, Cell Signaling, Cell Viability, Proliferation and Function, Immunoassays, Ready-To-Use Immunoassay