Macherey-Nagel™ NucleoSpin™ 8 PCR Clean-up, 8-well kit for PCR clean up

The NucleoSpin 8 Extract II kits allow the rapid clean-up of up to 15?g of DNA per preparation with high DNA recovery and reproducible yields. After purification with NucleoSpin 8 Extract II the highly pure PCR products are ready for immediate use in many applications, such as radioactive and fluorescent dye sequencing, including capillary sequencing, restriction analysis, ligation, and microarray analysis. NucleoSpin Extract II Binding Strips/Plates can be used under vacuum or by centrifugation. The NucleoSpin 8 Extract II kit uses advanced silica-membrane technology. The addition of a binding buffer containing chaotropic ions leads to a reversible adsorption of DNA to the NucleoSpin 8 Extract II silica membrane. PCR products bind to the silica membrane under optimised conditions, while contaminants like primers, salts, nucleotides, and proteins are completely removed by effective washing. Highly pure PCR products are eluted with low-salt elution buffer (5mM Tris/HCl pH8.5) or water (pH8.0 to 8.5) and are ready-to-use directly for subsequent applications.

Required hardware

Centrifugation

For centrifuge processing of NucleoSpin 8 Extract II kits a microtiterplate centrifuge is required which is able to accommodate the NucleoSpin Extract II Strips/Plate stacked on a round- or square-well block or rack with tube strips and reaches accelerations of 5,600 - 6,000 x g (bucket height: 85mm).For centrifuge processing of the 8-well strips the reusable starter set C, containing column holders C, dummy strips, MN square-well blocks, tube strips is required.

Vacuum processing

The NucleoSpin 8 Extract II kits can be used manually with the NucleoVac 96 vacuum manifold.For vacuum processing of the 8-well strips the reusable starter set A, containing column holders A and dummy strips is required.Kit components: NucleoSpin Extract binding strips, MN wash plate, racks with tube strips, cap strips, buffers.

• Silica membrane technology
• Format: 8-well strips
• Yield: 75% to 95%
• Sample material: <100μL PCR reaction mixture
• Fragment size: 65bp to 10kb
• Elution volume:75μL to 150μL
• Binding capacity: 15μg
• Preparation time: 30 min./6 strips
• Format: mini spin column

For clean-up of PCR* products.