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Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up, kit for gel extraction or PCR clean up
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N. di reazioni:
10 Preps
240 Preps
50 preparazioni
Dimensione della confezione:
10 reazioni
240 preparazioni
50 reazioni
Descrizione
NucleoSpin™ Gel and PCR Clean-up is designed for fast purification of PCR products, such as DNA from enzymatic reactions, as well as the extraction of DNA fragments from TAE or TBE agarose gels.
- Two applications in one kit—One buffer to process both sample types, with only one loading step
- High recovery for fragments down to 50 bp—Average recovery from 60–90%
- Minimized elution volume of 15μL—Highly concentrated DNA
- Binding buffer with pH indicator—Ensures optimal binding conditions
- Separate buffers for single stranded DNA and RNA or for SDS-containing samples available separately
- Suitable for all kinds of agarose gels and a variety of buffer systems (e.g. TAE, TBE)
- Complete removal of contaminations: nucleotides, primers, enzymes, mineral oil, PCR additives, detergents, dyes
- Applications: purification of PCR products, extraction of DNA from agarose or polyacrylamide gels, and downstream applications (cloning, sequencing, PCR, restriction analysis)
Kit contents: NucleoSpin™ Gel and PCR Clean-up columns, collection tubes (2mL), binding buffer with pH indicator, wash buffer and elution buffer
Specifica
Specifica
| Colore | pH Indicator: Yellow = correct conditions Green = pH slightly high (adjustment recommended) Blue = pH too high (adjustment necessary) |
| Formato | Mini Spin Column |
| Isolation Technology | Silica Membrane Technology |
| Efficienza | Typical recovery: 70–95% |
| Volume di eluizione | Elution volume: 15–30μL |
| Da utilizzare con (apparecchiatura) | TAE or TBE agarose gels, PCR |
| Include | Binding Buffer NTI (10mL), Wash Buffer NT3 Concentrate (6mL), Elution Buffer NE (13mL), NucleoSpin Gel and PCR Clean-up Columns (10), 2mL Collection Tubes (10) |
| N. di reazioni | 10 Preps |
| Quantità | 1 Kit |
| Purezza | A260/A280: 1.8–1.9 |
| Vedi altri risultati |
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