Macherey-Nagel™ NucleoTraPCR kit for PCR clean up

The NucleoTraP™CR extraction system is especially designed for direct purification of DNA fragments from PCR reactions without gel electrophoresis. DNA is reversibly adsorbed to the matrix with the aid of chaotropic agents. The NucleoTraP™CR extraction system can selectively bind DNA fragments ≥100bp, while the primer and primer-dimer molecules remain in solution. After two washing steps the DNA is eluted in low-salt elution buffer (5mM Tris/HCl, pH 8.5) or another low-salt medium (eg. water, pH 8.0). The eluted DNA shows a very high purity. All impurities such as enzymes, nucleotides and primer molecules are quantitatively removed. The eluted DNA can be used directly for all kinds of cloning experiments (ligation reactions, labelling reactions). DNA purified with NucleoTraP™CR gives excellent results in sequencing experiments using radioactive or fluorescent detection methods. Kit components: NucleoTraP™ suspension, buffers.

• Silica matrix technology
• Yield: 70% to 80%
• Sample material: 20μL to 400μL PCR reaction mixture
• Elution volume: 20μL to 50μL
• Binding capacity: 6μg/10μL suspension
• Preparation time: 45 min./6 preps
• Fragment size: 100bp to 20kb
• Format: silica bead suspension
• No use of organic solvents For direct purification of PCR* products.