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OPTIZYME™ DNase I (RNase-Free), Fisher BioReagents™
Marca: Fisher Bioreagents BP8107-1
Dettagli aggiuntivi : Peso : 0.02000kg
Descrizione
OPTIZYME™ DNase I is a recombinant endonuclease that digests DNA and is RNase-Free.
Applications and Features:
- OPTIZYME DNase I, RNase-Free is used for the preparation of DNA-free RNA
- Removal of template DNA following in vitro transcription
- DNA labeling by nick translation in conjunction with DNA Polymerase I
- Preparation of DNA-free RNA prior to RT-PCR
- Studies of DNA-protein interactions by DNase I, RNase-free footprinting
- Enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+or Mn2+ions
- Purified from non-animal host with a lower level of intrinsic RNases
Supplied with:
- 10X OPTIZYME DNase I Buffer: 100mM Tris-HCl (pH 7.5 at 25°C), 25mM MgCl2, 1mM CaCl2
- 50mM OPTIZYME EDTA (for inactivation by heat at 65°C for 10min)
Quality Control:
- Ribonuclease-free
- Functionally tested for the digestion of template DNA after in vitro transcription
Specifica
| 1 U/μL | |
| 7.5 | |
| OPTIZYME™ DNase I Buffer 10X : Tris HCl 100mM (7.5 pH at 25°C), MgCl2 25mM, CaCl2 1mM, OPTIZYME™ EDTA 50mM (for inactivation by heat at 65°C for 10min.) | |
| E. coli cells with a cloned gene encoding bovine DNase I | |
| DNase I (RNase-free) |
| Colorless | |
| Preparation of DNA-free RNA, removal of template DNA following in-vitro transcription, DNA labeling by nick translation in conjunction with DNA Polymerase I, Preparation of DNA-free RNA prior to RT-PCR, Studies of DNA-Protein interactions by DNase I, RNase-free footprinting | |
| -20°C | |
| 1000 U | |
| Liquid |