OPTIZYME™ T4 Polynucleotide Kinase, Fisher BioReagents™

OPTIZYME™ T4 PNK catalyzes the transfer of γ-phosphate from ATP to the 5'-OH group of DNA, RNA, oligonucleotides and nucleoside 3'-monophosphates.

Applications and Features:

• OPTIZYME™ T4 PNK labels 5'-termini of nucleic acids (for use as: probes for hybridization or transcript mapping, primers for PCR or DNA sequencing, and markers for gel-electrophoresis)
• 5'-phosphorylation of oligonucleotides, PCR products, and other DNA or RNA prior to ligation
• Phosphorylation of PCR primers
• Removal of 3'-phosphate groups
• Active in buffers for OPTIZYME restriction enzymes, T4 DNA Ligase and Reverse Transcriptases

Supplied With:

• 10X OPTIZYME T4 PNK Buffer 1 (for forward reaction): 500mM Tris-HCl (pH7.6 at 25°C), 100mM MgCl₂, 50mM DTT, 1mM spermidine
• 10X OPTIZYME T4 PNK Buffer 2 (for exchange reaction): 500mM imidazole-HCl (pH6.4 at 25°C), 180mM MgCl₂, 50mM DTT, 1mM spermidine, 1mM ADP
• 24% PEG 6000 Sol for T4 PNK: 24% (w/v) polyethylene glycol 6000

• Endo-, exodeoxyribonuclease and ribonuclease-free
• Functionally tested for labeling of 5'-termini of DNA