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OPTIZYME™ TaqI, Fisher BioReagents™
Marca: Fisher BioReagents BP8007-1
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Descrizione
5'...T^C G A...3'
3'...A G C^T...5'
Supplied with: 10X OPTIZYME TaqI Buffer
Conditions for 100% Activity:
- 1X OPTIZYME Buffer TaqI: 10mM Tris-HCl (pH 8.0 at 37°C), 5mM MgCl2, 100mM NaCl and 0.1mg/ml BSA
- Incubate at 65°C.
- To ensure higher efficiency digestion, perform the cleavage reaction under paraffin oil.
- Buffer 1: 0 - 20%
- Buffer 2: 20 - 50%
- Buffer 3: 20 - 50%
- Buffer 4: 20 - 50%
- Buffer 5: 20 - 50%
- 10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% (v/v) glycerol
- TaqI is blocked by overlapping dam methylation.
- To avoid dam methylation, use a dam-, dcm- strain.
- Incubation at 37°C results in 10% activity.
Methylation Effects:
Dam: May overlap - blocked
- 5'...TCGm6A TC...3'
- TCGA
Specifica
10 U/μL | |
65°C | |
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with TaqI | |
3000 U | |
Taql |
10X OPTIZYME™ TaqI Buffer | |
7.4 | |
10mM Tris HCl (pH 7.5 at 25°C), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 50% Glycerol | |
T.CGA | |
Liquid |