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OPTIZYME™ XbaI, Fisher BioReagents™
74.80€ - 221.00€
Specifica
| Componenti | 10X OPTIZYME™ Buffer 4 |
|---|---|
| Temperatura incubatore | 37°C |
| pH | 7.4 |
| Da utilizzare con (applicazione) | Indicates ligation efficiency of DNA fragments generated by digestion with the Restriction Enzyme |
| Tampone di conservazione | 10mM Tris HCl (pH 7.4), 100mM KCl, 7mM 2-mercaptoethano, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol |
| Codice del prodotto | Marca | Concentrazione | Quantità | Prezzo | Quantità e disponibilità | ||||
|---|---|---|---|---|---|---|---|---|---|
| Codice del prodotto | Marca | Concentrazione | Quantità | Prezzo | Quantità e disponibilità | ||||
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11984842
|
Fisher Bioreagents
BP8008-1 |
10 U/μL | 2000 U |
74.80€
Pezzo |
Spedizione stimata: 13-05-2024 Eseguire il login per visualizzare lo stock disponibile |
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|
11994842
|
Fisher Bioreagents
BP8008-5 |
50 U/μL | 10,000 U |
Questo articolo non è attualmente disponibile o è stato dismesso. La preghiamo di consultare la pagina del prodotto per individuare le possibili alternative. |
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Descrizione
5'...T^C T A G A...3'
3'...A G A T C^T...5'
Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity
- 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 50 - 100%
- Buffer 2: 50 - 100%
- Buffer 3: 20 - 50%
- Buffer 4: 100%
- Buffer 5: 0 - 20%
10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 7mM 2-mercaptoethanol, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
Ligation and Re-cleavage:
More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with XbaI.
Digestion of Agarose-embedded DNA:
A minimum 5 units of XbaI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Notes:
- XbaI is blocked by overlapping dam methylation.
- To avoid dam methylation, use a dam-, dcm- strain.
Methylation Effects:
Dam: May overlap - blocked
- 5'...TCTAGm6A TC...3'
- 3'...AGATC Tm6AG...5'
- (Cleavage is blocked)
CpG: Never overlaps - no effect
EcoKI: Never overlaps - no effect
EcoBI: Never overlaps - no effect
Specifica
| 10X OPTIZYME™ Buffer 4 | |
| 7.4 | |
| 10mM Tris HCl (pH 7.4), 100mM KCl, 7mM 2-mercaptoethano, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol | |
| XbaI |
| 37°C | |
| Indicates ligation efficiency of DNA fragments generated by digestion with the Restriction Enzyme | |
| T.CTAGA | |
| Liquid |