OPTIZYME™ XmnI (PdmI), Fisher BioReagents™

Name: OPTIZYME&trade; XmnI (PdmI), Fisher BioReagents&trade;
Brand: Fisher Bioreagents
SKU: 11914812
Price: 54.90 EUR

Marca: Fisher Bioreagents BP8052-1

Dettagli aggiuntivi : Peso : 0.01000kg

Codice prodotto. 11914812

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Descrizione

5'...G A A N N^N N T T C...3'
3'...C T T N N^N N A A G...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA.
Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

Buffer 1: 20 – 50%
Buffer 2: 50 - 100%
Buffer 3: 0 - 20%
Buffer 4: 100%
Buffer 5: 0 - 20%

Storage Buffer:

10mM Tris-HCl (pH 7.5 at 25°C), 100mM KCl, 1mM DTT, 5mM MgCl₂, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with XmnI

Digestion of Agarose-embedded DNA:

A minimum of 10 units of XmnI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.

Methylation Effects:

Dam: Never overlaps - no effect

Dcm: Never overlaps - no effect

CpG: May overlap - cleavage impaired

5'...GAA(N)₄TTm5C G...3'

3'...CTT(N)₄AA Gm5C...5'

(Cleavage is impaired)

5'...m5C GAA(N)₄TTm5C G...3'

'3... Gm5CTT(N)₄AA Gm5C...5'

(Cleavage is blocked)

EcoKI: May overlap - effect not determined

EcoBI: May overlap - effect not determined

Specifica

Concentrazione	10 U/μL
Temperatura incubatore	37°C
Da utilizzare con (applicazione)	>95% of DNA fragments can be ligated and re-cut after 50 fold over-digestion with XmnI
Quantità	500 U
Product Type	XmnI

Componenti	10X OPTIZYME™ Buffer 4
pH	7.4
Tampone di conservazione	10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 5mM MgCl2, 0.2mg/mL BSA, 50% Glycerol
Sito di taglio	GAANN.NNTCC
Forma	Liquid

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OPTIZYME™ XmnI (PdmI), Fisher BioReagents™

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