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Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, saturated with 100 mM Tris-EDTA to pH 8.0, for molecular
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Quantità:
100 mL
500 mL
2.5 L
Dimensione della confezione:
100mL
2.50L
500mL
Descrizione
This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
- This solution contains a mixture of pure chloroform, isoamyl alcohol and phenol saturated with Tris-EDTA, with a stabilized pH of 8.0
Applications
- Phenol-Chloroform-Isoamyl alcohol mixture solution (25:24:1) is used in the extraction of nucleic acids, DNA, and RNA
- When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.
- It is also used to generate DNA minisatellite fingerprints
Specifica
Specifica
| Materiale o nome chimico | Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, saturated with 100 mM Tris-EDTA to pH 8.0 |
| Colore | Yellow to Orange |
| Nota nome | DNase, RNase and Protease Free |
| CAS | 136112-00-0, 67-66-3, 123-51-3 |
| Formula molecolare | C6H6O |
| Numero MDL | MFCD00133763 |
| Numero UN | 2927 |
| Densità | 1.2800g/mL |
| Indice di Merck | 14, 7241 |
| Peso formulazione | 94.11 |
| Vedi altri risultati |
RUO – Research Use Only
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